Vicia villosa Lectin (VVL/VVA) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. Fluorescein conjugates display a high rate of photobleaching, signal is sensitive to pH changes, broad fluorescence emission spectrum, and fluorescence quenching on conjugation to biopolymers.

Source:
Hairy vetch

Activity:
≥250ug/ml is usally required to agglutinate type A1 cells.
≤1ug/ml will agglutinate neuraminidase cells.

Sugar Specificity:
N-Acetylgalactosamine

Inhibitory Carbohydrate:
N-Acetylgalactosamine

Divalent Ions:
Ca++, Mn++

Excitation:
495nm

Emission:
515nm

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.