TransScript® -Uni One-Step gDNA Removal and cDNA Synthesis SuperMix
Cat# AU311-03
Size : 100rxns×20ulsystem
Brand : TransGen Biotech
ManualTransScript®-Uni RT is an improved version of M-MLV reverse transcriptase with broad range of reaction temperature(42°C-65°C) and higher thermostability. The suggested reaction temperature is 50°C. The SuperMix contains reagents for simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85°C for 5 seconds.
• Broad range reaction temperature (42°C -65°C ) .
• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• cDNA up to 20 kb.
Applications
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
• cDNA library construction, primer extension, 3’ and 5’ RACE
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AU311-02 (50 rxns) | AU311-03 (100 rxns) |
| TransScript® -Uni RT/RI Enzyme Mix | 50 μl | 100 μl |
| gDNA Remover | 50 μl | 100 μl |
| 2×TS-Uni Reaction Mix | 500 μl | 1 ml |
| Random Primer(N9) (0.1 μg/μl) | 50 μl | 100 μl |
| Anchored Oligo(dT)20 Primer (0.5 μg/μl) | 50 μl | 100 μl |
| RNase-free Water | 500 μl | 1 ml |
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Lin J , Wu Y , Han B , et al. iTRAQ-based proteomic profiling of granulosa cells from lamb and ewe after superstimulation[J]. Theriogenology, 2017:S0093691X17302893.