TransScript® Probe One-Step qRT-PCR SuperMix
Cat# AQ221-01
Size : 100rxns×20μlsystem
Brand : TransGen Biotech
ManualTransScript® Probe One-Step qRT-PCR SuperMix is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers and fluorescent probes to achieve one step from reverse transcription to qPCR in a single tube.
Highlights
• Simple to use with reduced contamination
• High sensitivity and high specificity
Applications
• High-copy and low-copy gene detection
• Detection of RNA virus or trace amounts of RNA
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
| Component | AQ221-01 | AQ221-02 |
| TransScript® Probe One-Step RT/RI Enzyme Mix | 40 μl | 160 μl |
| TransStart® Probe qPCR SuperMix (2×) | 1 ml | 4×1 ml |
| Passive Reference Dye (50×) | 40 μl | 160 μl |
| RNase-free Water | 1 ml | 4×1 ml |
Li W, Mao L, Zhou B, et al. The swine CD81 enhances E2-based DNA vaccination against classical swine fever[J]. Vaccine, 2015, 33(30):3542-3548.