Total Bile Acid (TBA) Colorimetric Assay Kit
Cat# E-BC-K181-M-96T
Size : 96T
Brand : Elabscience
| Detection Principle | With S-NAD+ as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid. |
| Synonyms | TBA |
| Sample Type | Serum,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (400-410 nm,optimum wavelength: 405 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.36 μmol/L |
| Detection Range | 1.77-40 μmol/L |
| Precision | inter-assay CV: 3.7% | intra-assay CV: 3.4% |
| Assay Time | 25 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Dog serum | 1 |
| Mouse serum | 1 |
| Rat serum | 1 |
| Bovine serum | 1 |
| 10% mouse liver | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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