Thiobarbituric Acid Reactive Substances (TBARS) Colorimetric BioAssay™ Kit

Oxidative attack of essential cell components by reactive oxygen species has been associated with several human diseases, such as atherosclerosis, cardiovascular diseases, diabetes, liver disorders, and inflammatory rheumatic diseases. Thiobarbituric Acid Reactive Substances (TBARS) are low-molecular-weight end products (mainly malondialdehyde, MDA) that are formed during the decomposition of lipid peroxidation products. Increased levels of TBARS have been demonstrated in these diseases. Simple, direct and accurate assays for TBARS find wide applications in research and drug discovery. TBARS assay is based on the reaction of TBARS with thiobarbituric acid (TBA) to form a pink colored product. The color intensity at 535nm or fluorescence intensity at (lex/em=560nm/585nm) is directly proportional to TBARS concentration in the sample.||Key Features:|Sensitive and accurate. Linear detection range|Colorimetric Assay: 1-30uM MDA|Fluorometric Assay: 0.1-1.5uM MDA||Applications:|Direct Assays: Serum, plasma, urine, saliva and other biological samples|Drug Discovery/Pharmacology: Effects of drugs on TBARS.||Kit Components:|T3774-92A: TBA Reagent, 1x25ml|T3774-92B: Trichloroacetic Acid, 10%, 1x25ml|T3774-92C: 6M MDA Standard, 1x1vial||Storage and Stability:|Store all reagents at -20°C. Avoid freeze/thaw cycles. Stable for 6 months after receipt.