Reagents and instruments for immunology, cell biology and molecular biology.
 
> Tetramethylrhodamine Methyl Ester, Perchlorate

Tetramethylrhodamine Methyl Ester, Perchlorate

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Cat# 70017

Size : 25MG

Brand : Biotium


TMRM

TMRM (Tetramethylrhodamine methyl ester, perchlorate) and TMRE are preferred dyes for quantitative measurements of membrane potentials using the Nernst equation.

TMRM

Product Attributes

CAS number

115532-50-8
Probe cellular localization

Mitochondria
For live or fixed cells

For live/intact cells
Assay type/options

Real-time imaging
Cell permeability

Membrane permeant
Apoptosis/viability marker

Mitochondrial potential
Potential dependence

Mitochondrial potential-dependent
Colors

Red
Excitation/Emission

549/574 nm

Technology

Cellular Stains
Protocols
SDS
Supporting Documents

Product Description

TMRM (Tetramethylrhodamine methyl ester, perchlorate) and TMRE (70016) are preferred dyes for quantitative measurements of membrane potentials using the Nernst equation. The dyes do not form aggregates in cell membranes and have minimal interaction with membrane proteins. Thus, the transmembrane distribution of the dyes is directly related to the membrane potential according to the Nernst equation. We also offer TMRE, 2 mM in DMSO.

  • λEx/λEm (MeOH) = 548/573 nm
  • Red solid soluble in DMSO, DMF or EtOH
  • Store at 4°C and protect from light
  • C25H25ClN2O7
  • MW: 501
  • [115532-50-8]

References

1. Meth Cell Biol 38, 195 (1993).

2. Meth Cell Biol 30, 193 (1989).

3. Biophys J 56, 1053 (1989).

4. Biophys J 53, 785 (1988).

 

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