Suc-AAPF-pNA [70967-97-4]
Cat# HY-P2573-25mg
Size : 25mg
Brand : MedChemExpress
Suc-AAPF-pNA (Suc-Ala-Ala-Pro-Phe-pNA) is a chromogenic p-nitroanilide (pNA) substrate with the Km of 1.7 mM. Cleavage of Suc-AAPF-pNA releases 4-nitroaniline, which is yellow in colour and can be measured spectrophotometrically. Suc-AAPF-pNA can be used for the measurement of free and membrane-bound cathepsin G in human neutrophils.
For research use only. We do not sell to patients.
Custom Peptide Synthesis
Suc-AAPF-pNA Chemical Structure
CAS No. : 70967-97-4
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Description |
Suc-AAPF-pNA (Suc-Ala-Ala-Pro-Phe-pNA) is a chromogenic p-nitroanilide (pNA) substrate with the Km of 1.7 mM. Cleavage of Suc-AAPF-pNA releases 4-nitroaniline, which is yellow in colour and can be measured spectrophotometrically. Suc-AAPF-pNA can be used for the measurement of free and membrane-bound cathepsin G in human neutrophils[1][2][3]. |
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In Vitro |
The elastase activity is measured as activity against the substrate Suc-AAPF-Pna. Aliquots (10 μL) of SmCTF samples are incubated at 30 °C with 200 μM Suc-AAPF-pNA and the activity of cercarial elastase measured as abosorbance at 400 nm[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
624.64 |
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Formula |
C30H36N6O9 |
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CAS No. |
70967-97-4 |
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Appearance |
Solid |
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Color |
White to off-white |
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Sequence |
{Suc}-Ala-Ala-Pro-Phe-{pNA} |
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Sequence Shortening |
Suc-AAPF-pNA |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
Sealed storage, away from moisture and light, under nitrogen
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen) |
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Solvent & Solubility |
In Vitro:
DMSO : 100 mg/mL (160.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 99.87% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 1.6009 mL | 8.0046 mL | 16.0092 mL | 40.0231 mL |
5 mM | 0.3202 mL | 1.6009 mL | 3.2018 mL | 8.0046 mL | |
10 mM | 0.1601 mL | 0.8005 mL | 1.6009 mL | 4.0023 mL | |
15 mM | 0.1067 mL | 0.5336 mL | 1.0673 mL | 2.6682 mL | |
20 mM | 0.0800 mL | 0.4002 mL | 0.8005 mL | 2.0012 mL | |
25 mM | 0.0640 mL | 0.3202 mL | 0.6404 mL | 1.6009 mL | |
30 mM | 0.0534 mL | 0.2668 mL | 0.5336 mL | 1.3341 mL | |
40 mM | 0.0400 mL | 0.2001 mL | 0.4002 mL | 1.0006 mL | |
50 mM | 0.0320 mL | 0.1601 mL | 0.3202 mL | 0.8005 mL | |
60 mM | 0.0267 mL | 0.1334 mL | 0.2668 mL | 0.6671 mL | |
80 mM | 0.0200 mL | 0.1001 mL | 0.2001 mL | 0.5003 mL | |
100 mM | 0.0160 mL | 0.0800 mL | 0.1601 mL | 0.4002 mL |
Suc-AAPF-pNA Related Classifications
- Others