Ribonuclease A antibody (Peroxidase)
Cat# orb344918-25ul
Size : 25ul
Brand : Biorbyt
Ribonuclease A antibody (Peroxidase)
Catalog Number: orb344918
| Catalog Number | orb344918 |
|---|---|
| Category | Antibodies |
| Description | Ribonuclease A antibody (Peroxidase) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IP, WB |
| Reactivity | Bovine |
| Isotype | IgG |
| Immunogen | Ribonuclease A [Bovine Pancreas] |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:10,000 - 1:40,000, IP: 1:100, WB: 1:500 - 1:2,500 |
| Form/Appearance | Liquid (sterile filtered) |
| Purity | Ribonuclease A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine Pancreas]. Cross reactivity against Ribonuclease A from other tissues and species may occur but have not been specifically determined. |
| Conjugation | HRP |
| UniProt ID | P61823 |
| NCBI | AAI49530.1 |
| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Alternative names | rabbit anti-Ribonuclease A Antibody Peroxidase Con |
| Note | For research use only |
| Application notes | Anti-Ribonuclease A Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Ribonuclease A [Bovine Pancreas] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. |
| Expiration Date | 12 months from date of receipt. |
Western Blot of Rabbit anti-Ribonuclease A Antibody Peroxidase Conjugated. Lane 1: Ribonuclease A (Bovine Pancreas). Load: 50 ng per lane. Primary antibody: Rabbit anti-Ribonuclease A Antibody Peroxidase Conjugated at 1:1000 overnight at 4°C. Secondary antibody: n/a. Block: orb348637 for 30 min at RT. Predicted/Observed size: 16.5 kDa, 16.5 kDa for Ribonuclease A.