Product Information

Product Type

Recombinant Protein

Expression System

E.coli

Domain

117-269aa

UniProt No.

P01584

NCBI Accession No.

NP_000567.1

Alternative names

Interleukin-1 beta, IL-1 beta, Catabolin, IL1F2

Product Specification

Molecular Weight

17 kDa (154aa) confirmed by MALDI-TOF

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 12pg/ml.

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Biological Activity

Human IL-1 beta /IL-1F2 stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range ≤ 12 pg/ml.

Related Publications

Choi DY, et al. Molecularly imprinted polymer-based electrochemical impedimetric sensors on screen-printed carbon electrodes for the detection of trace cytokine IL-1β. (Biosens Bioelectron. 2022)
Park RW, et al. Mobile Point-of-Care Device Using Molecularly Imprinted Polymer-Based Chemosensors Targeting Interleukin-1β Biomarker. (Biosensors. 2023)