Nitric Oxide (NO) Colorimetric Assay Kit
Detection principle
Performance characteristics
Synonyms | NO |
Sample type | Serum, plasma, tissue, saliva |
Sensitivity | 0.16 μmol/L |
Detection range | 0.16-100 μmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 55 min |
Precision | Average inter-assay CV: 3.700%Average intra-assay CV: 2.400% |
Other instruments required | Micropipettor, Vortex mixer, Centrifuge |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | Reagent 6: -20℃, others: 2-8℃ |
Valid period | 12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.16-100 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type | Dilution factor |
Human serum | 1 |
Human plasma | 1 |
10% Mouse liver tissue homogenization | 1 |
Rat serum | 1 |
Rat plasma | 1 |
10% Epipremnum aureum tissue homogenization | 1 |
Note: The diluent is double distilled water, normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Troubleshooting
Collection and treatment of common samples for metabolism assay detection
Questions and Answers of Metabolism Assay Kits
Citations
-
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 5.924
Parathyroid Hormone Induces Human Valvular Endothelial Cells Dysfunction That Impacts the Osteogenic Phenotype of Valvular Interstitial CellsDOI: 10.3390/ijms23073776
PMID: 35409134
Sample: Valvular Endothelial Cell -
Frontiers in Pharmacology (2022) IF: 5.988
Anti-arthritic and toxicological evaluation of ethanolic extract of Alternanthera bettzickiana in rats.Sample: Serum -
FOOD AND CHEMICAL TOXICOLOGY (2021) IF: 6.025
Reactive astrocytes induced by 2-chloroethanol modulate microglia polarization through IL-1β, TNF-α, and iNOS upregulationPMID: 34517076
Sample: Astrocyte -
Frontiers in Pharmacology (2021) IF: 5.811
Identification of the Antithrombotic Mechanism of Leonurine in Adrenalin Hydrochloride-Induced Thrombosis in Zebrafish via Regulating Oxidative Stress and Coagulation Cascade. -
Journal of Clinical Tuberculosis and Other Mycobacterial Diseases (2021)
Phagocytosis and the antigen-processing abilities of macrophages derived from monocytes in spinal tuberculosis patientsSample: Macrophages -
Advances in Traditional Medicine (2020)
Anti-hypernociceptive effects of methanol extract of Boswellia dalzielii on STZ-induced diabetic neuropathic painSample: Serum,Sciatic Nerves -
MOLECULAR BIOLOGY REPORTS (2020) IF: 1.402
Codeine-induced hepatic injury is via oxido-inflammatory damage and caspase-3-mediated apoptosisPMID: 33211294
Sample: Lvier -
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2019) IF: 1.645
Adventitious root cultures of Oplopanax elatus inhibit LPS-induced inflammation via suppressing MAPK and NF-κB signaling pathwaysSample: Pms Cells -
Journal of Obsessive-Compulsive and Related Disorders (2021) IF: 1.677
TGF-β1, neopterin, tetrahydrobiopterin, and nitric oxide levels in pediatric obsessive–compulsive disorderSample: Serum -
BIOMEDICAL AND ENVIRONMENTAL SCIENCES (2019) IF: 1.917
Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient MiceDOI: 10.3967/bes2018.101
Sample: Plasma,Tissue Homogenate -
ANDROLOGIA (2020) IF: 1.951
Low androgen status inhibits erectile function by up‐regulating the expression of P2X receptors in rat corpus cavernosumDOI: 10.1111/and.13627
Sample: Corpus Cavernosum -
Veterinary Sciences (2021) IF: 2.304
Protective and Therapeutic Effects of Orlistat on Metabolic Syndrome and Oxidative Stress in High-Fat Diet-Induced Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) in Rats: Role on Nrf2 ActivationSample: Liver -
JOURNAL OF MOLECULAR HISTOLOGY (2021) IF: 2.611
Salivary exosomes as a new therapy to ameliorate diabetes mellitus and combat xerostomia and submandibular salivary glands dysfunction in diabetic ratsPMID: 33389429
Sample: Serum -
DNA AND CELL BIOLOGY (2018) IF: 2.634
GPR30 Attenuates Myocardial Fibrosis in Diabetic Ovariectomized Female Rats: Role of iNOS SignalingPMID: 30227089
Sample: Serum -
Iranian Journal of Basic Medical Sciences (2021) IF: 2.699
Corilagin potential in inhibiting oxidative and inflammatory stress in LPS-induced murine macrophage cell lines (RAW 264.7)Sample: Raw 264.7 Cell -
ACS Omega (2020) IF: 2.87
Antiarthritic Potential of Comprehensively Standardized Extract of Alternanthera bettzickiana: In Vitro and In Vivo StudiesPMID: 32803042
Sample: Serum -
Molecular Medicine Reports (2021) IF: 2.952
α‑rhamnrtin‑3‑α‑rhamnoside exerts anti‑inflammatory effects on lipopolysaccharide‑stimulated RAW264.7 cells by abrogating NF‑κB and activating the Nrf2 signaling pathwayPMID: 34523697
Sample: Raw264.7 Cell -
JOURNAL OF ETHNOPHARMACOLOGY (2018) IF: 3.115
Anti-inflammatory action of Athyrium multidentatum extract suppresses the LPS-induced TLR4 signaling pathwaySample: Peritoneal Macrophages (Pms) -
Animals (2022) IF: 3.231
Expression of Hormones, Cytokines, and Antioxidants in Heat-Stressed Subfertile Female DromedariesDOI: 10.3390/ani12162125
PMID: 36009715
Sample: Serum -
BEHAVIOURAL BRAIN RESEARCH (2022) IF: 3.352
Prenatal chronic stress impairs the learning and memory ability via inhibition of the NO/cGMP/PKG pathway in the Hippocampus of offspringPMID: 35850398
Sample: Hippocampal Tissue -
Andrology (2022) IF: 3.842
Low androgen status inhibits erectile function by upregulating the expression of proteins of mitochondria-associated membranes in rat corpus cavernosumDOI: 10.1111/andr.13188
PMID: 35466583
Sample: Penile Cavernous Tissue -
Andrology (2021) IF: 3.842
Low androgen status inhibits erectile function by increasing pyroptosis in rat corpus cavernosumDOI: 10.1111/andr.12995
PMID: 33657666
Sample: Cell Lysate -
Andrology (2022) IF: 3.842
Low androgen level impairs erectile function of rat by regulating the Ng/CaN/AKT/eNOS pathway in penile corpus cavernosumDOI: 10.1111/andr.13202
PMID: 35678097
Sample: Penile Cavernous Tissue -
ACS Omega (2022) IF: 4.132
Ajuga bracteosa Exerts Antihypertensive Activity in l-NAME-Induced Hypertension Possibly through Modulation of Oxidative Stress, Proinflammatory Cytokines, and the Nitric Oxide/Cyclic Guanosine Monophosphate PathwayPMID: 36157749
Sample: Serum -
Food Bioscience (2021) IF: 4.24
Auricularia polytricha and Flammulina velutipes ameliorate inflammation and modulate the gut microbiota via regulation of NF-κB and Keap1/Nrf2 signaling pathways on DSS-induced inflammatory bowel diseaseSample: Colon,Liver -
JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
Ziziphus Oxyphylla hydro-methanolic extract ameliorates hypertension in L-NAME induced hypertensive rats through NO/cGMP pathway and suppression of oxidative stress related inflammatory biomarkers.Sample: Serum -
JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
Aronia melanocarpa (Michx.) Elliott. attenuates dextran sulfate sodium-induced Inflammatory Bowel Disease via regulation of inflammation-related signaling pathways and modulation of the gut microbiotaPMID: 35306040
Sample: Liver Homogenates -
Journal of Functional Foods (2021) IF: 4.451
Structural characterization and immunomodulatory activity of a glucan from Radix CodonopsisSample: Cell Culture Supernatant -
Andrology (2022) IF: 4.456
Icariin inhibits the formation of mitochondria-associated membranes (MAMs) and improves erectile function in rats treated with prostate radiationDOI: 10.1111/andr.13218
PMID: 35765205
Sample: Penile Cavernous Tissue -
MOLECULES (2023) IF: 4.927
Neuroprotective Effects of Savinin on LPS-Induced Neuroinflammation In Vivo via Regulating MAPK/NF-κB Pathway and NLRP3 Inflammasome ActivationSample: Hippocampa -
INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
Daurisoline alleviated experimental colitis in vivo and in vitro: Involvement of NF-κB and Wnt/β-Catenin pathwayPMID: 35366641
Sample: Colon Tissues,Raw 264.7 Cell -
Marine Drugs (2021) IF: 5.118
MMP-9 and IL-1β as Targets for Diatoxanthin and Related Microalgal Pigments: Potential Chemopreventive and Photoprotective AgentsDOI: 10.3390/md19070354
PMID: 34206447
Sample: Hacat Cells,B16-F0 Cells -
Nutrients (2022) IF: 5.719
Paraxanthine Supplementation Increases Muscle Mass, Strength, and Endurance in MiceDOI: 10.3390/nu14040893
PMID: 35215543
Sample: Serum -
Frontiers in Pharmacology (2022) IF: 5.811
Effects of Pereskia aculeate Miller Petroleum Ether Extract on Complete Freund’s Adjuvant-Induced Rheumatoid Arthritis in Rats and its Potential Molecular MechanismsSample: Raw264.7 -
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
Bee Bread Ameliorates Vascular Inflammation and Impaired Vasorelaxation in Obesity-Induced Vascular Damage Rat Model: The Role of eNOS/NO/cGMP-Signaling PathwayDOI: 10.3390/ijms22084225
PMID: 33921777
Sample: Tissue Homogenate(Aortic ) -
Antioxidants (2021) IF: 6.313
Hepatoprotective Effect of Bee Bread in Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) Rats: Impact on Oxidative Stress and InflammationPMID: 34943134
Sample: Liver -
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 6.53
Gastroprotective effect of gallic acid against ethanol-induced gastric ulcer in rats: Involvement of the Nrf2/HO-1 signaling and anti-apoptosis rolePMID: 32179202
Sample: Tissue Homogenate -
BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
D-ribose-L-cysteine prevents oxidative stress and cardiometabolic syndrome in high fructose high fat diet fed ratsPMID: 34399203
Sample: Heart -
Oxidative Medicine and Cellular Longevity (2021) IF: 6.543
Leonurine Ameliorates Oxidative Stress and Insufficient Angiogenesis by Regulating the PI3K/Akt-eNOS Signaling Pathway in H2O2-Induced HUVECsDOI: 10.1155/2021/9919466
Sample: Huvecs -
HYPERTENSION (2018) IF: 6.823
Potential Protective Mechanism in the Cardiac Microvascular InjurySample: Cardiac Microvascular Endothelial Cells (Cmecs) -
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021) IF: 6.953
Structural characterization and immunomodulatory activities of two polysaccharides from Rehmanniae Radix PraeparataPMID: 34197855
Sample: Raw264.7 Cells -
Frontiers in Immunology (2021) IF: 7.561
Transcriptional Profiling and Functional Analysis of N1/N2 Neutrophils Reveal an Immunomodulatory Effect of S100A9-Blockade on the Pro-Inflammatory N1 SubpopulationSample: Neutrophils -
Biomaterials Science (2022) IF: 7.59
Transdermal delivery of allopurinol on acute hyperuricemic mice via polymer microneedles for regulation of serum uric acid levelsDOI: 10.1039/D2BM01836E
Sample: Serum -
Antioxidants (2022) IF: 7.675
Therapeutic Effects of Heterotrigona itama (Stingless Bee) Bee Bread in Improving Hepatic Lipid Metabolism through theActivation of the Keap1/Nrf2 Signaling Pathway in an Obese Rat ModelSample: Liver -
SCIENCE OF THE TOTAL ENVIRONMENT (2022) IF: 7.963
Cold exposure, gut microbiota, and hypertension: A mechanistic studyPMID: 35417730
Sample: Plasma