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> Mouse IgG (H&L) antibody (TRITC)

Mouse IgG (H&L) antibody (TRITC)

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Cat# orb347494-2mg

Size : 2mg

Brand : Biorbyt


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    Mouse IgG (H&L) antibody (TRITC)

    Catalog Number: orb347494

    Catalog Numberorb347494
    CategoryAntibodies
    DescriptionMouse IgG (H&L) antibody (TRITC)
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsDOT, FC, FLISA, IF, WB
    ReactivityMouse
    IsotypeIgG
    ImmunogenMouse IgG whole molecule
    Concentration2.0 mg/mL
    Dilution rangeFLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
    Form/AppearanceLyophilized
    PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG, and Mouse Serum.
    ConjugationTRITC
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesRabbit Anti-Mouse IgG Antibody rhodamine Conjugati
    NoteFor research use only
    Application notesAnti-Mouse IgG Rhodamine Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Expiration Date12 months from date of receipt.
    Mouse IgG (H&L) antibody (TRITC)

    Fluorescence-activated cell sorting analysis after co-icubation of HCT116 cells with FITC-labeled CaIX-P1 and rhodamine-labeled anti-human CAIX mAb. Low left box: unlabeled cells (autofluorescence). Low right box: cells labeled only with FITC-CaIX-P1. Upper left box: cells labeled only with rhodamine-anti-CAIX-mAb. Upper right box: Cells labeled with both FITC-CaIX-P1 and rhodamine-anti-CAIX-mAb.

    Mouse IgG (H&L) antibody (TRITC)

    Western blot of Rhodamine conjugated Rabbit Anti-Mouse IgG secondary antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine rabbit secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.