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> Meta-topolin [75737-38-1]

Meta-topolin [75737-38-1]

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Cat# HY-112104-50mg

Size : 50mg

Brand : MedChemExpress


Meta-Topolin (m-Topolin) is a highly active aromatic cytokinin.

For research use only. We do not sell to patients.

Meta-topolin Chemical Structure

Meta-topolin Chemical Structure

CAS No. : 75737-38-1

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Description

Meta-Topolin (m-Topolin) is a highly active aromatic cytokinin.

In Vitro

Meta-Topolin is first isolated from poplar leaves. Meta-Topolin is a natural constituent of plant tissues, together with its 9-β-D-ribofuranosyl and 9-β-v-glucopyranosyl derivatives. Meta-topolin is more active than zeatin and benzyladenine in the promotion of shoot formation in plant tissue cultures[1]. In Spathiphyllum floribundum, shoot production in media with BAP and meta-topolin is very similar[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Meta-topolin Related Antibodies
Molecular Weight

241.25

Formula

C12H11N5O
CAS No.

75737-38-1
Appearance

Solid

Color

White to off-white

SMILES

OC1=CC=CC(CNC2=C3N=CNC3=NC=N2)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (518.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1451 mL 20.7254 mL 41.4508 mL
5 mM 0.8290 mL 4.1451 mL 8.2902 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (8.62 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (8.62 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.55%

COA (249 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

  • [1]. Strnad M, et al. Meta-topolin, a highly active aromatic cytokinin from poplar leaves (Populus × canadensis Moench., cv. Robusta). Phytochemistry 45(2):213-218.

    [2]. Werbrouck S, et al. Meta-topolin, an alternative to benzyladenine in tissue culture? 1996. 98 (2):291-297.

  • [1]. Strnad M, et al. Meta-topolin, a highly active aromatic cytokinin from poplar leaves (Populus × canadensis Moench., cv. Robusta). Phytochemistry 45(2):213-218.

    [2]. Werbrouck S, et al. Meta-topolin, an alternative to benzyladenine in tissue culture? 1996. 98 (2):291-297.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.1451 mL 20.7254 mL 41.4508 mL 103.6269 mL
5 mM 0.8290 mL 4.1451 mL 8.2902 mL 20.7254 mL
10 mM 0.4145 mL 2.0725 mL 4.1451 mL 10.3627 mL
15 mM 0.2763 mL 1.3817 mL 2.7634 mL 6.9085 mL
20 mM 0.2073 mL 1.0363 mL 2.0725 mL 5.1813 mL
25 mM 0.1658 mL 0.8290 mL 1.6580 mL 4.1451 mL
30 mM 0.1382 mL 0.6908 mL 1.3817 mL 3.4542 mL
40 mM 0.1036 mL 0.5181 mL 1.0363 mL 2.5907 mL
50 mM 0.0829 mL 0.4145 mL 0.8290 mL 2.0725 mL
60 mM 0.0691 mL 0.3454 mL 0.6908 mL 1.7271 mL
80 mM 0.0518 mL 0.2591 mL 0.5181 mL 1.2953 mL
100 mM 0.0415 mL 0.2073 mL 0.4145 mL 1.0363 mL
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Meta-topolin Related Classifications

  • Others
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Help & FAQs

Keywords:

Meta-topolin75737-38-1m-TopolinOthersInhibitorinhibitorinhibit

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