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> Magnesium (Mg) Colorimetric Assay Kit

Magnesium (Mg) Colorimetric Assay Kit

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Cat# E-BC-K162-M-500

Size : 500Assays

Brand : Elabscience


Detection principle

The magnesium in the serum reacts with the complexometric indicator (Calmagite) to form the Calmagite-Mg compound. The absorbance of this compound at 540 nm is proportional to the concentration of magnesium in the sample. The concentration of magnesium can be calculated by measuring the OD value at 540 nm.

Performance characteristics

Synonyms Mg
Sample type Serum,plasma
Sensitivity 0.18 mmol/L
Detection range 0.18-2.50 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 20 min
Precision Average inter-assay CV: 7.900%Average intra-assay CV: 5.100%
Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.18-2.50 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Rat serum

1

Mouse serum

1

Porcine serum

1

Chicken serum

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

  1. Frontiers in Plant Science (2022) IF: 6.627
    Halotolerant Bacillus altitudinis WR10 improves salt tolerance in wheat via a multi-level mechanism

    DOI: 10.3389/fpls.2022.941388

    PMID: 35909740

    		 Sample: Roots Or Shoots