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Iron is essential for nearly all living organisms and is generally stored in the heme complex, in the centre of metalloproteins and in oxygen carrier proteins. Inorganic iron is also involved in redox reactions in the iron-sulfur clusters of many enzymes, such as hydrogenase and nitrogenase.
The Iron Colorimetric Assay Kit provides a simple, convenient and highly sensitive way for detection of Ferric ion and/or Ferrous concentration in various biological samples based on colorimetric method. In the assay, ferric carrier protein dissociates ferric into solution in the presence of acid buffer. After reduction to Fe2+, iron reacts with Ferene S to generate a stable colored (λmax = 593 nm) complex that can be easily qualified using a microtiter plate reader or a spectrophotometer at 593 nm. A specific chelate chemical in the buffer is used to block copper ion (Cu2+) interference. The assay detects iron in the linear range of 8 µM to 400 µM iron concentration in various samples.
