IgG is the predominant immunoglobulin in the serum and has a molecular weight of about 150kD. Four distinct subgroups of human IgG (IgG1, IgG2, IgG3, and IgG4) were first demonstrated in the 1960’s by using polyclonal antisera prepared in animals immunized with human myeloma proteins. Quantitatively, the relative serum concentrations of the IgG subclasses are as follows: IgG1 > IgG2 > IgG3 = IgG4. The four IgG subclasses show their most conspicuous differences in the amino acid composition and structure of the 'hinge region', which is the part of the molecule containing disulfide bonds between the gamma-heavy chains. The hinge region of IgG1 encompasses aa216-231 and since it is freely flexible, the Fab fragments can rotate about their axes of symmetry and move within a sphere centered at the first of two inter-heavy chain disulfide bridges.||Applications: |Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.||Recommended Dilutions:|ELISA: 1:100-1:5000 |Western Blot: 1:50-400|Immunohistochemistry (frozen): 1:50-500|Immunohistochemistry (paraffin): 1:10-100|Immunocytochemistry: 1:50-500|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.