HSL/LIPE Polyclonal Antibody
Cat# bs-22905R
Size : 100ul
Brand : Bioss
HSL/LIPE Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HSL\/LIPE Polyclonal Antibody, Unconjugated (bs-22905R) at 1:200 overnight at 4\u00b0C, DAB staining.
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-22905R |
| Product Name | HSL/LIPE Polyclonal Antibody |
| Applications | IHC-P |
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HSL/LIPE |
| Immunogen Range | 441-540/1076 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 3991 |
| Swiss Prot | Q05469 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Hormone sensitive lipase; hormone-sensitive lipase; Hormone sensitive lipase testicular isoform; HSL; LHS; Lipase hormone sensitive; LIPE; Hormone-sensitive lipase; LIPS_HUMAN. |
| Background | In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. |
| Application Dilution | |
| IHC-P | 1:200-400 |