- More Files
Western Blot (Tissue lysate)
Western blot analysis of Aqp2 in rat kidney extracts using Aqp2 polyclonal antibody (Cat # PAB12114). Key : CX=Cortex, OM=Outer Medulla, IMB =Inner Medulla Base, IMP = Inner Medulla Papilla.
Immunocytochemistry
Staining of Aqp2 on mpkCCD cell line using Aqp2 polyclonal antibody (Cat # PAB12114).
- Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Aqp2.
Immunogen
A synthetic peptide corresponding to residues within amino acids 200-300 at C-terminus of rat Aqp2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Immunogen affinity purification
Recommend Usage
Immunocytochemistry/Immunofluorescence (1 ug/mL)
Immunohistochemistry (1:100-1:200)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:200)
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This antibody is useful for western blot, where bands can be seen ~28 kDa for the non-glycosylated form and ~37 kDa for the glycosylated form. - Applications
Western Blot (Tissue lysate)
Western blot analysis of Aqp2 in rat kidney extracts using Aqp2 polyclonal antibody (Cat # PAB12114). Key : CX=Cortex, OM=Outer Medulla, IMB =Inner Medulla Base, IMP = Inner Medulla Papilla.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Staining of Aqp2 on mpkCCD cell line using Aqp2 polyclonal antibody (Cat # PAB12114). - Gene Info — Aqp2