Anti-RIPK3 antibody (468-518) {Biotin}

Clonality:	Polyclonal
Conjugation:	Biotin
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.66-0.68 µg/µl
Dilution Range:	WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:200
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	RIPK3
Gene ID:	11035
Uniprot ID:	RIPK3_HUMAN
Immunogen Region:	468-518
Immunogen:	Synthetic peptide taken within amino acid region 468-518 on human Receptor-interacting Serine/Threonine-protein kinase 3 protein.

Tissue Specificity	Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3: Expression is significantly increased in colon and lung cancers.
Post Translational Modifications	(Microbial infection) Proteolytically cleaved by S.flexneri OspD3 within the RIP homotypic interaction motif (RHIM), leading to its degradation and inhibition of necroptosis. RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. Autophosphorylated following interaction with ZBP1. Phosphorylation of Ser-199 plays a role in the necroptotic function of RIPK3. Autophosphorylates at Ser-227 following activation by ZBP1: phosphorylation at these sites is a hallmark of necroptosis and is required for binding MLKL. Phosphorylation at Thr-182 is important for its kinase activity, interaction with PELI1 and PELI1-mediated 'Lys-48'-linked polyubiquitination and for its ability to mediate TNF-induced necroptosis. Polyubiquitinated with 'Lys-48' and 'Lys-63'-linked chains by BIRC2/c-IAP1 and BIRC3/c-IAP2, leading to activation of NF-kappa-B. Polyubiquitinated with 'Lys-48'-linked chains by PELI1 leading to its subsequent proteasome-dependent degradation. Ubiquitinated by STUB1 leading to its subsequent proteasome-dependent degradation.
Function	Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death. Necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members, is triggered by RIPK3 following activation by ZBP1. Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Also regulates apoptosis: apoptosis depends on RIPK1, FADD and CASP8, and is independent of MLKL and RIPK3 kinase activity. Phosphorylates RIPK1: RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. In some cell types, also able to restrict viral replication by promoting cell death-independent responses. In response to Zika virus infection in neurons, promotes a cell death-independent pathway that restricts viral replication: together with ZBP1, promotes a death-independent transcriptional program that modifies the cellular metabolism via up-regulation expression of the enzyme ACOD1/IRG1 and production of the metabolite itaconate. Itaconate inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production. (Microbial infection) In case of herpes simplex virus 1/HHV-1 infection, forms heteromeric amyloid structures with HHV-1 protein RIR1/ICP6 which may inhibit RIPK3-mediated necroptosis, thereby preventing host cell death pathway and allowing viral evasion.
Protein Name	Receptor-Interacting Serine/Threonine-Protein Kinase 3 Rip-Like Protein Kinase 3 Receptor-Interacting Protein 3 Rip-3
Database Links	Reactome: R-HSA-168927 Reactome: R-HSA-1810476 Reactome: R-HSA-2562578 Reactome: R-HSA-3295583 Reactome: R-HSA-5213460 Reactome: R-HSA-5675482 Reactome: R-HSA-9013957 Reactome: R-HSA-937041 Reactome: R-HSA-9686347 Reactome: R-HSA-9692913 Reactome: R-HSA-9692916
Cellular Localisation	Cytoplasm Cytosol Nucleus Mainly Cytoplasmic Present In The Nucleus In Response To Influenza A Virus (Iav) Infection
Alternative Antibody Names	Anti-Receptor-Interacting Serine/Threonine-Protein Kinase 3 antibody Anti-Rip-Like Protein Kinase 3 antibody Anti-Receptor-Interacting Protein 3 antibody Anti-Rip-3 antibody Anti-RIPK3 antibody Anti-RIP3 antibody

Information sourced from Uniprot.org

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