Reagents and instruments for immunology, cell biology and molecular biology.

Anti-CYB5R1 antibody (1-305)

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Cat# NB-22-72075-100

Size : 100uL

Brand : Neo Biotech

Immunohistochemistry analysis of CYB5R1 in paraffin-embedded Rat ovary using CYB5R1 Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of CYB5R1 in paraffin-embedded Human esophageal using CYB5R1 Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Western blot analysis of various lysates using CYB5R1 Rabbit polyclonal antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

General Info

Host:	Rabbit
Applications:	WB/IHC
Reactivity:	Human/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-CYB5R1 (1-305) is suitable for use in Western Blot and Immunohistochemistry research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IHC-P 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	CYB5R1
Gene ID:	51706
Uniprot ID:	NB5R1_HUMAN
Immunogen Region:	1-305
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human CYB5R1 (NP_057327.2).
Immunogen Sequence:	MGIQTSPVLLASLGVGLVTL LGLAVGSYLVRRSRRPQVTL LDPNEKYLLRLLDKTTVSHN TKRFRFALPTAHHTLGLPVG KHIYLSTRIDGSLVIRPYTP VTSDEDQGYVDLVIKVYLKG VHPKFPEGGKMSQYLDSLKV GDVVEFRGPSGLLTYTGKGH FNIQPNKKSPPEPRVAKKLG MIAGGTGITPMLQLIRAILK VPEDPTQCFLLFANQTEKDI ILREDLEELQARYPNRFKL

Description

Tissue Specificity	Widely expressed.
Function	NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
Protein Name	Nadh-Cytochrome B5 Reductase 1 B5r.1 Humb5r2 Nad(Ph -Quinone Oxidoreductase Type 3 Polypeptide A2
Database Links	Reactome: R-HSA-114608 Reactome: R-HSA-1237044
Cellular Localisation	Membrane Single-Pass Membrane Protein
Alternative Antibody Names	Anti-Nadh-Cytochrome B5 Reductase 1 antibody Anti-B5r.1 antibody Anti-Humb5r2 antibody Anti-Nad(Ph -Quinone Oxidoreductase Type 3 Polypeptide A2 antibody Anti-CYB5R1 antibody Anti-NQO3A2 antibody Anti-UNQ3049 antibody Anti-PRO9865 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance