Aflatoxin B1 [1162-65-8]
Cat# HY-N6615-5mg
Size : 5mg
Brand : MedChemExpress
Aflatoxin B1 (AFB1) is a Group 1A carcinogen and a secondary metabolite produced by Aspergillus flavus and A. parasiticus. Aflatoxin B1 (AFB1) primarily induces mutations by causing a G to T transversion at the third nucleotide of codon 249 in the tumor suppressor gene p53. In the body, Aflatoxin B1 (AFB1) can cause damage to the intestines and kidneys and has some teratogenic effects.
For research use only. We do not sell to patients.
Aflatoxin B1 Chemical Structure
CAS No. : 1162-65-8
This product is a controlled substance and not for sale in your territory.
Based on 4 publication(s) in Google Scholar
Other Forms of Aflatoxin B1:
- Aflatoxin B1 (Standard) Get quote
- Aflatoxin B1-13C17 Get quote
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Description |
Aflatoxin B1 (AFB1) is a Group 1A carcinogen and a secondary metabolite produced by Aspergillus flavus and A. parasiticus. Aflatoxin B1 (AFB1) primarily induces mutations by causing a G to T transversion at the third nucleotide of codon 249 in the tumor suppressor gene p53. In the body, Aflatoxin B1 (AFB1) can cause damage to the intestines and kidneys and has some teratogenic effects[1][2][3][4][5][6]. |
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IC50 & Target |
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In Vitro |
Aflatoxin B1 (0-1 μg/mL; 24-72 h) dose- and time-dependently inhibits the growth of differentiated and undifferentiated Caco-2 cells and increases DNA damage and ROS accumulation in Caco-2 cells[3]. Aflatoxin B1 (0-200 mg/L; 48 h) inhibits HEK 293 cell viability and induces apoptosis[4]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Cell Cytotoxicity Assay[3]
Cell Cytotoxicity Assay[4]
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In Vivo |
Aflatoxin B1 (0.5 mg/kg; i.g.; once a day for 28 days) causes significant damage to the renal tissue of mice[4]. Aflatoxin B1 (0-0.1 mg/kg; i.g.; administered at gestational days 6-18) is teratogenic in New Zealand white rabbits[5]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Molecular Weight |
312.27 |
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Formula |
C17H12O6 |
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CAS No. |
1162-65-8 |
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Appearance |
Solid |
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Color |
White to yellow |
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SMILES |
COC1=CC(O[C@]2(07)[C@@]3(07)C=CO2)=C3C(O4)=C1C(CCC5=O)=C5C4=O |
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Initial Source |
Aspergillus flavus;A. parasiticus |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
-20°C, protect from light *In solvent : -80°C, 1 year; -20°C, 6 months (protect from light) |
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Solvent & Solubility |
In Vitro:
DMF : 33.33 mg/mL (106.73 mM; Need ultrasonic) DMSO : 30 mg/mL (96.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 99.96% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO / DMF | 1 mM | 3.2024 mL | 16.0118 mL | 32.0236 mL | 80.0589 mL |
5 mM | 0.6405 mL | 3.2024 mL | 6.4047 mL | 16.0118 mL | |
10 mM | 0.3202 mL | 1.6012 mL | 3.2024 mL | 8.0059 mL | |
15 mM | 0.2135 mL | 1.0675 mL | 2.1349 mL | 5.3373 mL | |
20 mM | 0.1601 mL | 0.8006 mL | 1.6012 mL | 4.0029 mL | |
25 mM | 0.1281 mL | 0.6405 mL | 1.2809 mL | 3.2024 mL | |
30 mM | 0.1067 mL | 0.5337 mL | 1.0675 mL | 2.6686 mL | |
40 mM | 0.0801 mL | 0.4003 mL | 0.8006 mL | 2.0015 mL | |
50 mM | 0.0640 mL | 0.3202 mL | 0.6405 mL | 1.6012 mL | |
60 mM | 0.0534 mL | 0.2669 mL | 0.5337 mL | 1.3343 mL | |
80 mM | 0.0400 mL | 0.2001 mL | 0.4003 mL | 1.0007 mL | |
DMF | 100 mM | 0.0320 mL | 0.1601 mL | 0.3202 mL | 0.8006 mL |
Aflatoxin B1 Related Classifications
- Induced Disease Models Products
- Cancer Models Immunology and Inflammatory Disease Models
- Liver Cancer Models Hapten
- Infection Cancer
- Cancer Immunotherapy Cancer Metabolism and Metastasis
- Apoptosis NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
- Apoptosis Reactive Oxygen Species Bcl-2 Family Caspase