3,3'-Diaminobenzidine tetrahydrochloride (DAB) is a substrate for peroxidases, especially for horseradish peroxidase (HRP).
DAB is used for histology and ultrahistochemistry (1) and demonstration of ultrastructural peroxidase (2).
It serves as a hydrogen donor in the presence of peroxide in the peroxidase reaction. The oxidized DAB forms an insoluble brown end-product, which can be detected in visible light and does not bleach during long-term storage.
Stock solution: 10 - 20 mg/ml in 50 mM Tris buffer, pH 7.3 (store in aliquots at -20 °C), working solution: 0.5 - 1 mg/ml in buffer (PBS, TBS, pH 7.0 - 7.6) containing 0.01 % hydrogen peroxide.
Vials under argon.

Assay (HPLC)

min. 96.0 %

References:

1. Hanker, J.S. et al. (1972) Histochemie 30, 201-14
2. Graham, R.C. & Karnovsky, M.J. (1966) J. Histochem. Cytochem. 14, 291- 302

DANGER

Hazard Statements	H341 - H350
Precautions	P201 - P280
Reaction	P308 +P313
Storage	P405

Carc. 1B, Muta. 2 • EINECS: 231-018-9 • WGK: 3 • HS: 29215990
Storage Temperature: +2 °C to +8 °C