α-Amylase and β-Amylase Activity Assay Kit
Cat# E-BC-K006-M-500
Size : 500Assays
Brand : Elabscience
| Detection Principle | The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance. Amylase activity can be calculated by measuring the OD value at 540 nm. |
| Sample Type | Serum,Plasma,Saliva,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (535-540 nm, optimum wavelength: 540 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.008 U/mL |
| Detection Range | 0.01-0.37 U/mL |
| Precision | inter-assay CV: 3.6% | intra-assay CV: 2.8% |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human plasma | 10-15 |
| Human serum | 10-15 |
| Human saliva | 15-25 |
| Mouse serum | 15-25 |
| Rat serum | 15-25 |
| 1% Corn grain tissue homogenate | 2-5 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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