Tubulin alpha antibody (Biotin)
Cat# orb345885-100ug
Size : 100ug
Brand : Biorbyt
Tubulin alpha antibody (Biotin)
Catalog Number: orb345885
| Catalog Number | orb345885 |
|---|---|
| Category | Antibodies |
| Description | Tubulin alpha antibody (Biotin) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC, WB |
| Reactivity | Bovine, Gallus, Goat, Human, Mouse, Rat, Sheep |
| Isotype | IgG |
| Immunogen | Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin. |
| Concentration | 0.61 mg/ml |
| Dilution range | ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, IF: 1:500 - 1:2,000, WB: 1:2,000-1:10,000 |
| Form/Appearance | Lyophilized |
| Purity | Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein. The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control. |
| Conjugation | Biotin |
| UniProt ID | P68363 |
| Storage | Store Anti-Tubulin Loading Control Antibody at 4° C prior to restoration. For extended storage aliquot Control Antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge Tubulin Antibody if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | rabbit anti-alpha-Tubulin Antibody biotin conjugat |
| Note | For research use only |
| Application notes | Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract. |
| Expiration Date | 12 months from date of receipt. |
ELISA Results of Purified Rabbit Anti-Alpha Tubulin Antibody Biotin Conjugated tested against purified Alpha Tubulin Biotin Conjugated. Each well was coated in duplicate with 0.1 µg of BSA conjugated Alpha Tubulin. The working dilution is 1:11000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit IgG HRP conjugated (p/n orb347654), and TMB substrate (p/n orb348651).