Sudan Black B [4197-25-5]

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Cat# HY-D0213-5g

Size : 5g

Brand : MedChemExpress

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Sudan Black B, a fat-soluble diazo dye, is a histochemical stain. Sudan Black B can be used for staining of neutral triglycerides and lipids.

For research use only. We do not sell to patients.

Sudan Black B Chemical Structure

Sudan Black B Chemical Structure

CAS No. : 4197-25-5

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Description

Sudan Black B, a fat-soluble diazo dye, is a histochemical stain. Sudan Black B can be used for staining of neutral triglycerides and lipids[1].

In Vitro

Sudan Black B works also in frozen material, and therefore can be complementarily used with SA-β-gal when investigating senescence. Sudan Black B is a highly lipophilic agent that exhibits high affi nity to the lipid compartment of lipofuscin. The positive Sudan Black B-lipofuscin reaction reveals blue-black intracellular granules in cells and frozen tissues and brown to black granules in FFPE tissues under the light microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

456.55

Formula

C29H24N6

CAS No.

4197-25-5

Appearance

Solid

Color

Brown to black

SMILES

CC(NC1=CC=CC2=C13)(C)NC3=CC=C2/N=N/C(C4=C5C=CC=C4)=CC=C5/N=N/C6=CC=CC=C6

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 1 mg/mL (2.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1903 mL 10.9517 mL 21.9034 mL
5 mM --- --- ---
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.13%

References
  • [1]. Konstantinos Evangelou, et al. Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells. Methods Mol Biol. 2017;1534:111-119.  [Content Brief]

  • [1]. Konstantinos Evangelou, et al. Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells. Methods Mol Biol. 2017;1534:111-119.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1903 mL 10.9517 mL 21.9034 mL 54.7585 mL
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Sudan Black B Related Classifications

  • Others
  • Others
  • Fluorescent Dye
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sudan Black B4197-25-5Fluorescent DyeFat-soluble diazo dyehistochemical staintriglycerideslipidsInhibitorinhibitorinhibit

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