SARS-CoV-2 Nucleocapsid Protein Monoclonal Antibody (14B2D)
Cat# bsm-41415M
Size : 100ul
Brand : Bioss
SARS-CoV-2 Nucleocapsid Protein Monoclonal Antibody (14B2D)
Lane 1: SARS-CoV-2 N protein(Cat# bs-41408P) at 50ng; Lane 2: SARS-CoV-2 N protein(Cat# bs-41408P) at 25ng; Lane 3: SARS-CoV-2 N protein(Cat# bs-41408P) at 5ng; Lane 4: SARS-CoV-2 N protein(Cat# bs-41408P) at 2ng ; Lane 5: SARS-CoV-2 N protein(Cat# bs-41408P) at 1ng;Lane 6: SARS-CoV-2 N protein(Cat# bs-41408P) at 0.5ng probed with SARS-CoV-2 N protein monoclonal Antibody, Unconjugated (bsm-41415M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Applications
Reactivity
| Overview | |
| Catalog # | bsm-41415M |
| Product Name | SARS-CoV-2 Nucleocapsid Protein Monoclonal Antibody (14B2D) |
| Applications | WB, ELISA |
| Reactivity | Virus |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone # | 14B2D |
| Isotype | IgG2b |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M PBS(pH7.4) with 0.1% Proclin300. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Swiss Prot | P0DTC9 |
| Synonyms | SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 peak N Protein; 2019-nCoV nucleocapsid protein. |
| Background | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
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