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Western Blot (Recombinant protein)
Application

Western Blot (Recombinant protein)

RUB1 polyclonal antibody (Cat # PAB11355), generated by immunization with full-length, recombinant yeast RUB1, was tested by immunoblot against a yeast cell lysate.
A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free RUB1 protein (indicated by arrow) and RUB1 conjugates.
In this blot, the antibody was used at a 1:500 dilution and was incubated overnight at 4°C in 5% non-fat dry milk in TTBS.
Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection (Roche).

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against full length recombinant RUB1.

    Immunogen

    Recombinant protein corresponding to full length Saccharomyces cerevisiae RUB1.

    Host

    Rabbit

    Reactivity

    Yeast

    Form

    Lyophilized

    Quality Control Testing

    Antibody Reactive Against Recombinant Protein.

    Recommend Usage

    ELISA (1:1000-1:5000)
    Western Blot (1:500)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot after reconstitution to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Recombinant protein)

    RUB1 polyclonal antibody (Cat # PAB11355), generated by immunization with full-length, recombinant yeast RUB1, was tested by immunoblot against a yeast cell lysate.
    A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free RUB1 protein (indicated by arrow) and RUB1 conjugates.
    In this blot, the antibody was used at a 1:500 dilution and was incubated overnight at 4°C in 5% non-fat dry milk in TTBS.
    Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
    A chemiluminescence system was used for signal detection (Roche).

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — RUB1

    Entrez GeneID

    851717

    Gene Name

    RUB1

    Gene Alias

    -

    Gene Description

    Rub1p

    Gene Ontology

    Hyperlink

    Gene Summary

    Rtt101p

    Other Designations

    Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)

  • Publication Reference