Retinoic acid [302-79-4]
Cat# T1051-1mL
Size : 1mL
Brand : TargetMol
Retinoic acid
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Purity:99.52%
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COA HNMR HPLC
Retinoic acid
Catalog No. T1051Cas No. 302-79-4
Retinoic acid (Tretinoin), a metabolite of vitamin A, is a natural agonist of the retinoic acid receptor RAR and inhibits RARα/β/γ (IC50=14 nM). Retinoic acid induces cellular differentiation, reduces cellular proliferation, and inhibits tumorigenesis.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose. Pack Size | |
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50 mg | |
100 mg | |
1 mL x 10 mM (in DMSO) |
Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
Description | Retinoic acid (Tretinoin), a metabolite of vitamin A, is a natural agonist of the retinoic acid receptor RAR and inhibits RARα/β/γ (IC50=14 nM). Retinoic acid induces cellular differentiation, reduces cellular proliferation, and inhibits tumorigenesis. |
Targets&IC50 | RARα:14 nM, RARβ:14 nM, RARγ:14 nM |
In vitro | Tretinoin prevents skin atrophy induced by corticosteroids in hairless mice. When co-administered with miquimod in guinea pigs, tretinoin induces tattoo fading and moderate pigment clearance histopathologically. Applications of tretinoin on incisions in the skin of 45 CD-1 mice increase fibroblast differentiation and reduce collagen production. In aged male Fischer 344 rats treated with tretinoin, renal cortex protein content is 30% lower compared to controls, potentially due to suppressed expression of tumor necrosis factor-β1 and osteopontin. |
In vivo | In studies evaluating the impact on glutathione levels and catalase activity, Tretinoin increased both metrics in a time- and dose-dependent manner, offering protective and mitigating effects against H2O2 cytotoxicity in human renal mesangial cells. Treatment with Tretinoin resulted in elevated mRNA levels of catalase and γ-glutamylcysteine synthetase (the catalytic subunit responsible for the rate-limiting step in reduced glutathione synthesis) in cultured mesangial cells. Additionally, Tretinoin upregulated matrix metalloproteinase-8/13 in human keloid-derived fibroblasts. |
Cell Research | Retinoic acid is dissolved in DMSO and stored, and then diluted with appropriate medium before use[3]. P19 cell are induced to undergo neuronal differentiation according to established procedures. Briefly, cells are cultured on 1% agarose-coated 10 cm dishes at 3×10 5 cells/mL in α-minimal essential medium supplemented with 10% FBS. Differentiation is induced by addition of Retinoic acid (1 μM) and medium containing Retinoic acid replaced 2 days later. On day 4, cell aggregates are collected by centrifugation, separated to single cells by trypsin/EDTA treatment, replated onto poly-L-lysine-coated plates, and cultured in α-minimal essential medium supplemented with 10% FBS. On day 6, medium is replaced with neurobasal medium containing B27 supplement and 2 mM GlutaMAX. Medium is replaced every 2 days for an additional week[3]. |
Alias | Vitamin A acid, ATRA, all-trans-Retinoic acid, Tretinoin |
Molecular Weight | 300.44 |
Formula | C20H28O2 |
Cas No. | 302-79-4 |
Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | ||||||||||||||||||||||||||||||||||||||||
Solubility Information | DMSO: 45 mg/mL (149.78 mM) Ethanol: 6 mg/mL (19.97 mM) H2O: < 1 mg/mL (insoluble or slightly soluble) | ||||||||||||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||||||||||||
Ethanol/DMSO
DMSO
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Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSO
main solution, add 300 μLPEG300
mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O
mix well and clarify
Dose Conversion
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
PPARinhibitEndogenous MetaboliteRAR/RXRInhibitorRetinoic acidRetinoic acid receptorsRetinoid X receptorsPeroxisome proliferator-activated receptorsAutophagy
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