PerfectStart™ II Probe qPCR SuperMix is a ready-to-use qPCR cocktail containing all components, except probe, primer and template. It contains PerfectStart™ Taq DNA Polymerase (PerfectStart™ Taq DNA polymerase is a hot-start Taq DNA polymerase containing Taq DNA polymerase and three kinds of monoclonal antibodies, effectively blocking DNA polymerase activity and preventing non-specific amplification at low temperature), specially optimized dual-cation buffer, dNTPs, PCR enhancer and stabilizer. qPCR SuperMix is provided at 2x concentration and can be used at 1x concentration by adding template, primer, probe, passive reference dye (optional) and Nuclease-free Water. dUTP/UDG is included in the kit to degrade dU-containing ssDNA and dsDNA, which can prevent cross contamination. 

Highlights

• PerfectStart™ Taq DNA Polymerase, hot start with three antibodies blocking technique, improves sensitivity, enhances specificity and generates more accurate data.

• Specially optimized qPCR reaction buffer provides higher sensitivity and specificity.

• dUTP/UDG used to avoid cross contamination.

• Passive reference dyes are provided for different qPCR instruments.

Storage

-20°C for two years

Shipping

Dry ice (-70°C)