WESTAR NOVA 2.0 is an ECL substrate with stable light output for low picogram detection level.
The formulation provides a low background for a high signal to noise ratio.
NeoPRO Pico Western ECL Substrate
Cat# NB-78-00002
Size : 2x125mLkit
Brand : Neo Biotech
Properties for WESTAR NOVA 2.0
| Description | WESTAR NOVA 2.0 is an ECL substrate with stable light output for low picogram detection level. The formulation provides a low background for a high signal to noise ratio. |
| Features | Low picogram detection Ideal for routinary analysis Working solution stable for at least three days The best entry level ECL substrate on the market |
| Components | Luminol/enhancer solution (A) Peroxide solution (B) |
| Antibody Dilution Range | Primary: 1:500 - 1:5.000 Secondary: 1:20.000 - 1:100.000 (from 1 mg/mL stock solution) |
| Available as | 2 x 125 mL kit (2500 cm2 of membrane) |
| Signal duration | 8 hours |
| Storage | Stable for 1 year at RT Product is shipped at ambient temperature |
Online Resources & Supporting Documents
Gallery
Western blotting detection of HDAC-1 on Hela cell lysate with WESTAR NOVA 2.0 and its competitors.
Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 5 μg to 0.078 μg of total protein
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Biorad)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:2000
Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1:20000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare)
Exposure time: 180 seconds
Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 5 μg to 0.078 μg of total protein
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Biorad)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:2000
Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1:20000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare)
Exposure time: 180 seconds
WESTAR NOVA 2.0 Signal Duration Comparison with Competitors.
Comparison of signal intensities at time points up to 20 hours post substrate addition.
Exposure time is 180 seconds for each time point (0-2-5-8 hours).
Comparison of signal intensities at time points up to 20 hours post substrate addition.
Exposure time is 180 seconds for each time point (0-2-5-8 hours).