NEFL (Neurofilament Light, 68kD Neurofilament Protein, CMT1F, CMT2E, NF L, NFL)

The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits. Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central alpha-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament. While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly. Neurofilament accumulations are found in many human neurological disorders including Parkinson's disease (in Lewy bodies along with alpha-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS).||Applications: |Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|Western Blot: 1:500-1:2000|Immunofluorescence: 1:50-1:200|Immunohistochemistry (Paraffin): 1:50-1:200|Optimal dilutions to be determined by the researcher.||Storage and Stability: |May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.