MOUSE IgG3 antibody (ATTO 425)
Cat# orb347442-500ug
Size : 500ug
Brand : Biorbyt
MOUSE IgG3 antibody (ATTO 425) [Out of stock]
Catalog Number: orb347442
ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
ATTO 425 conjugated anti-Mouse IgG was used to demonstrate 2 color STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 µg/mL final concentration of anti-a-tubulin and detected with 0.2 µg/mL ATTO 425 conjugated anti-MOUSE IgG [GOAT] (orb347443) secondary antibody (colored RED). Also shown in this 2-color STED image is Biorbyt's Anti-HDAC-1 [RABBIT] (p/n orb345508) detected with DyLight™488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody (colored GREEN).