Human Mesenchymal Stem Cells, Endometrium
Cat# 300647
Size : 1millioncells
Brand : CLS Cell Lines Service
Human Mesenchymal Stem Cells - Endometrium
General information
Description | MSCs, or multipotent mesenchymal stromal cells, are self-renewing multipotent cells that can differentiate into a wide variety of cell types. The in vitro direct differentiation of MSCs into at least three orthodoxal lineages-adipocytes, osteoblasts, and chondrocytes has been demonstrated. Using differentiation media, it is possible to differentiate cultivated MSCs into adipocytes, osteoblasts, and chondrocytes in vitro. Early passage cultured MSCs are cryopreserved using a specific cryomedium.?After thawing, each cryovial contains 1 x 10^6 (minimum 92% to 95% viability level by Trypan Blue dye exclusion test). The MSCs were collected from healthy donors which have given their informed consent for the donation of the cell material. Each batch of MSCs is subjected to strict quality control testing (both cell donors and cell cultures). The identification, purity, potency, viability, and appropriateness of cultured MSCs for the intended usage are all evaluated. |
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Organism | Human |
Tissue | Endometrium |
Applications | Drug testing, regenerative medicine, disease research |
Characteristics
Age | Please inquire |
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Gender | Please inquire |
Ethnicity | Caucasian |
Morphology | Well-spread spindle shaped, fibroblast-like morphology for at least within 5 passages. Fewer than 2% cells exhibit spontaneous myofibroblast-like morphology within each passage. |
Cell type | Stem cell |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | Human Mesenchymal Stem Cells, Whartons Jelly (HMSC-WJ) (Cytion catalog number 300685) |
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Biosafety level | 1 |
Expression / Mutation
Antigen expression | A comprehensive panel of markers, including CD73/CD90/CD105 (positive) and CD14/CD34/CD45/HLA-DR (negative), are used in flow cytometry analysis to identify cultivated MSCs (P2-P3) prior to cryopreservation. These markers are recommended by the ISCT MSC committee. |
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Viruses | Donor is negative for HBV (PCR), Treponema pallidum (PCR), and HIV-1/2 (IFA). Cells are negative for HBV, HCV, HSV1, HSV2, CMV, EBV, HHV6, Toxoplasma gondii, Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, and Ureaplasma parvum. |
Handling
Culture Medium | Alpha MEM, w: 2.0 mM stable Glutamine, w/o: Ribonucleosides, w/o: Deoxyribonucleosides, w: 1.0 mM Sodium pyruvate, w: 2.2g/L NaHCO3 |
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Medium supplements | Supplement the medium with 10% FBS, 2 ng/mL bFGF |
Passaging solution | Trypsin-EDTA |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Seeding density | 1 to 3 x 10^4 cells/cm^2 |
Fluid renewal | First fluid renewal after 24 hours, then every 2 to 3 days. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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