Images
Flow Cytometry
Application

Flow Cytometry

Flow cytometric analysis of HeLa cells with HSPB1 (phospho S82) monoclonal antibody, clone CB2 (APC) (Cat # MAB21649). Unstained and untreated cells as as negative control (blue) or untreated (red) or treated with UV and TPA (green).

  • Specifications

    Product Description

    Rabbit monoclonal antibody raised against synthetic phosphopeptide of human HSPB1.

    Immunogen

    A synthetic phosphopeptide corresponding to residues surrounding S82 of human HSPB1.

    Host

    Rabbit

    Reactivity

    Human

    Form

    Liquid

    Conjugation

    APC

    Purification

    Protein A/G purification

    Isotype

    IgG1, kappa

    Recommend Usage

    Flow Cytometry (5 uL/106 cells)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.4 (0.2% BSA, 0.09% sodium azide).

    Storage Instruction

    Store at 4°C.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Flow Cytometry

    Flow cytometric analysis of HeLa cells with HSPB1 (phospho S82) monoclonal antibody, clone CB2 (APC) (Cat # MAB21649). Unstained and untreated cells as as negative control (blue) or untreated (red) or treated with UV and TPA (green).
  • Gene Info — HSPB1

    Entrez GeneID

    3315

    Protein Accession#

    P04792

    Gene Name

    HSPB1

    Gene Alias

    CMT2F, DKFZp586P1322, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27

    Gene Description

    heat shock 27kDa protein 1

    Omim ID

    602195 606595 608634

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq

    Other Designations

    OTTHUMP00000024846|estrogen-regulated 24 kDa protein|heat shock 27kD protein 1|heat shock protein beta-1|stress-responsive protein 27

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