HS-683 genomic DNA - 5 microgram
Cat# 300213GD5
Size : 5ug
Brand : CLS Cell Lines Service
HS-683 Cells
General information
Description | HS-683 is a human glioma cell line derived from the brain tissue of an adult patient diagnosed with glioblastoma multiforme. Glioblastoma multiforme is a highly aggressive type of brain cancer, known for its rapid growth and poor prognosis. The HS-683 cell line is valuable in cancer research due to its ability to provide insights into the molecular mechanisms driving glioma proliferation, invasion, and resistance to therapies. HS-683 cells exhibit many characteristics typical of glioma cells, including high proliferative capacity and the expression of markers such as GFAP (glial fibrillary acidic protein), which is indicative of their glial origin. These cells are commonly used in studies investigating the efficacy of chemotherapeutic agents, radiation treatments, and novel targeted therapies. Researchers utilize HS-683 to explore genetic and epigenetic alterations, signal transduction pathways, and the tumor microenvironment's role in glioma progression. The HS-683 cell line, therefore, serves as a crucial model for developing and testing new therapeutic strategies aimed at improving outcomes for patients with glioblastoma. |
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Organism | Human |
Tissue | Brain |
Disease | Oligodendroglioma |
Synonyms | HS 683, Hs 683, Hs-683, Hs683, HS683, Hs 683.T, HS 683T, Hs683T |
Characteristics
Age | 76 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Fibroblast-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | HS-683 (Cytion catalog number 300213) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | G6PD, B, PGM1, 1, PGM3, 1-2, ES-D, 1, Me-2, 2, AK-1, 1, GLO-1, 2, Phenotype Frequency Product: 0.0029 |
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Tumorigenic | No |
Ploidy status | Aneuploid |
Karyotype | (P15) hypotetraploid with mode = 88, range = 44 to 97, Y chromosomes present |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 45 to 50 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 is recommended |
Seeding density | When seeded at 1 x 10^4 cells/cm^2 the cells will reach 80% confluence within 3 to 4 d. |
Fluid renewal | Every 3 days |
Freezing recovery | After thawing, plate the cells at 4 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,y CSF1PO: 9,13 D13S317: 8,12 D16S539: 9,10 D5S818: 11,12 D7S820: 11 TH01: 6,8 TPOX: 8,11 vWA: 18,20 D3S1358: 14,16 D21S11: 27,33.2 D18S51: 12,14 Penta E: 13,15 Penta D: 13,14 D8S1179: 12,13 FGA: 21.2,22 |
HLA alleles | A*: 32:01:01 B*: 07:02:01, 44:02:01 C*: 05:01:01, 07:02:01 DRB1*: 08:01:01, 12:01:01 DQA1*: 04:01:01, 05:05:01 DQB1*: 03:01:01, 04:02:01 DPB1*: 02:01:02, 03:01:01 E: 01:01:01 |