Hoechst 33342, 10mg/mL in H2O

Cat# 40046

Size : 10ML

Brand : Biotium

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Hoechst

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains.

Technology

Cellular Stains
Considerations for Immunofluorescence Staining

Tech Tips

Hoechst & DAPI Staining Protocols – Cell Staining with Hoechst or DAPI Nuclear Stains

Product Description

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. These dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains. Biotium offers Hoechst 33258 and Hoechst 33342 dyes in both solution or powder forms. Both dyes are spectrally similar but Hoechst 33258 is slightly more water soluble than Hoechst 33342. The dyes can be used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required.

Hoechst can also be used to stain live bacteria (gram-positive and gram-negative), but in live yeast the staining is weak and not nuclear. Check out our tech tip on Hoechst & DAPI Staining, which includes helpful tips and detailed protocols for staining live and fixed cells and tissues. Also see our Cellular Stains Table for more information on how our dyes stain various organisms.

Hoechst 33258, pentahydrate

 

Hoechst 33342, trihydrochloride trihydrate

Biotium also offers unique NucSpot® Nuclear Stains for bright and specific nuclear staining in dead or fixed cells. The stains are available in a wide range of colors from green to near-IR. We also offer DAPI nuclear stains in powder or solution.

Hoechst Products

Product Catalog Number MW Unit Size λEx/λEm Format
Hoechst 33258, 10 mg/mL in Water 40044 533.88 10 mL 352/458 nm Yellow solution
Hoechst 33258, pentahydrate 40045 623.96 100 mg 352/458 nm Yellow solid
Hoechst 33342, 10 mg/mL in Water 40046 561.93 10 mL 350/461 nm Yellow solution
Hoechst 33342, trihydrochloride trihydrate 40047 615.98 100 mg 350/461 nm Yellow solid

Having trouble with your experiment? See our section on troubleshooting tips for fluorescent staining.

 

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Cat#
Description
Cond.
Price Bef. VAT
8488-SC
 100ug