GPR68 antibody
Cat# orb256569-100ul
Size : 100ul
Brand : Biorbyt
Item 1 of 2
Item 1 of 2
GPR68 antibody
Catalog Number: orb256569
| Catalog Number | orb256569 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to GPR68 |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, WB |
| Reactivity | Human, Mouse, Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPR68. The exact sequence is proprietary. |
| Dilution range | WB: 1:500-1000, IF/ICC: 1:100-500 |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Conjugation | Unconjugated |
| Target | GPR68 |
| Entrez | 8111, 238377 |
| UniProt ID | Q8BFQ3, Q15743 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Alternative names | anti OGR1 antibody, anti Ovarian cancer G-protein |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of GPR68 expression in mouse muscle (A), mouse testis (B), rat testis (C) whole cell lysates. (Predicted band size: 41 kD; Observed band size: 41 kD)
Immunofluorescent analysis of GPR68 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).