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Western Blot (Tissue lysate)
Application

Western Blot (Tissue lysate)

GOPC polyclonal antibody (Cat # PAB6562, 0.05 ug/mL) staining of human frontal cortex lysate (35 ug protein in RIPA buffer) . Primary incubation was 1 hour. Detected by chemiluminescence.

  • Specifications

    Product Description

    Goat polyclonal antibody raised against synthetic peptide of GOPC.

    Immunogen

    A synthetic peptide corresponding to human GOPC.

    Sequence

    C-LDDLHTLYHKKSY

    Host

    Goat

    Theoretical MW (kDa)

    50.5, 49.7

    Reactivity

    Human

    Specificity

    This antibody is expected to recognize both reported isoforms (NP_065132.1, NP_001017408.1).

    Form

    Liquid

    Purification

    Antigen affinity purification

    Concentration

    0.5 mg/mL

    Quality Control Testing

    Antibody Reactive Against Synthetic Peptide.

    Recommend Usage

    ELISA (1:128000)
    Western blot (0.05-0.2 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Tissue lysate)

    GOPC polyclonal antibody (Cat # PAB6562, 0.05 ug/mL) staining of human frontal cortex lysate (35 ug protein in RIPA buffer) . Primary incubation was 1 hour. Detected by chemiluminescence.

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — GOPC

    Entrez GeneID

    57120

    Protein Accession#

    NP_065132.1;NP_001017408.1

    Gene Name

    GOPC

    Gene Alias

    CAL, FIG, GOPC1, PIST, dJ94G16.2

    Gene Description

    golgi associated PDZ and coiled-coil motif containing

    Omim ID

    606845

    Gene Ontology

    Hyperlink

    Gene Summary

    PIST is a PDZ domain-containing Golgi protein. PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner.[supplied by OMIM

    Other Designations

    CFTR-associated ligand|Golgi associated PDZ and coiled-coil motif containing protein|OTTHUMP00000017088|OTTHUMP00000040403|PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10|dJ94G16.2 PIST|fused in glioblastoma

  • Interactomes
  • Publication Reference