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Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to α-ketoglutarate and is present in the mitochondria of eukaryotes and most microbes. Since increased serum GDH levels indicate liver damage, GDH often used as an indicator of liver disease, often in combination with aminotransferases.
The Glutamate Dehydrogenase Activity Colorimetric Assay Kit provides a sensitive, simple, fast and convenient way for detection of GDH activity in various samples based on colorimetric method. In the assay, GDH in sample consumes glutamate as a specific substrate and produces NADH stoichiometrically, resulting in a proportional color development that can be easily quantified colorimetrically (λmax = 450 nm). The kit can detect GDH activity as low as 0.01 mU in serum or tissue and cell extracts.
