- More Files
Western Blot (Tissue lysate)
The DAPK1 polyclonal antibody (Cat # PAB2049) is used in Western blot to detect DAPK1 in mouse liver tissue lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with DAPK1 polyclonal antibody (Cat # PAB2049) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DAPK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DAPK1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Concentration
0.25 mg/mL
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot (Tissue lysate)
The DAPK1 polyclonal antibody (Cat # PAB2049) is used in Western blot to detect DAPK1 in mouse liver tissue lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with DAPK1 polyclonal antibody (Cat # PAB2049) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. - Gene Info — DAPK1
Entrez GeneID
1612Protein Accession#
NP_004929;P53355Gene Name
DAPK1
Gene Alias
DAPK, DKFZp781I035
Gene Description
death-associated protein kinase 1
Omim ID
600831Gene Ontology
HyperlinkGene Summary
Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. [provided by RefSeq
Other Designations
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- Interactomes
- Pathways
- Diseases
- Publication Reference
- The pro-apoptotic function of death-associated protein kinase is controlled by a unique inhibitory autophosphorylation-based mechanism.
Shohat G, Spivak-Kroizman T, Cohen O, Bialik S, Shani G, Berrisi H, Eisenstein M, Kimchi A.
The Journal of Biological Chemistry 2001 Dec; 276(50):47460.
- Death-associated protein kinase-related protein 1, a novel serine/threonine kinase involved in apoptosis.
Inbal B, Shani G, Cohen O, Kissil JL, Kimchi A.
Molecular and Cellular Biology 2000 Feb; 20(3):1044.
- Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death.
Deiss LP, Feinstein E, Berissi H, Cohen O, Kimchi A.
Genes & Development 1995 Jan; 9(1):15.
Application：WB-Ce, WB-Re, WB-Tr, Human, Monkey, COS-7, HeLa cells, Purified protein.
- The pro-apoptotic function of death-associated protein kinase is controlled by a unique inhibitory autophosphorylation-based mechanism.