Cholesterol [57-88-5]
Cat# HY-N0322-500mg
Size : 500mg
Brand : MedChemExpress
Cholesterol is the major sterol in mammals. It is making up 20-25% of structural component of the plasma membrane. Plasma membranes are highly permeable to water but relatively impermeable to ions and protons. Cholesterol plays an important role in determining the fluidity and permeability characteristics of the membrane as well as the function of both the transporters and signaling proteins. Cholesterol is also an endogenous estrogen-related receptor α (ERRα) agonist.
For research use only. We do not sell to patients.
Cholesterol Chemical Structure
CAS No. : 57-88-5
This product is a controlled substance and not for sale in your territory.
Based on 28 publication(s) in Google Scholar
Other Forms of Cholesterol:
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- Cholesterol (Standard) In-stock
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- Cholesterol-d6 In-stock
- Cholesterol-d6-1 In-stock
- Cholesterol-13C2 In-stock
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View All Estrogen Receptor/ERR Isoform Specific Products:
Description |
Cholesterol is the major sterol in mammals. It is making up 20-25% of structural component of the plasma membrane. Plasma membranes are highly permeable to water but relatively impermeable to ions and protons. Cholesterol plays an important role in determining the fluidity and permeability characteristics of the membrane as well as the function of both the transporters and signaling proteins[1][2]. Cholesterol is also an endogenous estrogen-related receptor α (ERRα) agonist[3]. |
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IC50 & Target |
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In Vitro |
GT1-7 hypothalamic cells subjected to Cholesterol depletion in vitro produced 20-31% reductions in cellular Cholesterol content. All Cholesterol-depleted neuron-derived cells, exhibit decreased phosphorylation/activation of IRS-1 and AKT following stimulation by insulin, insulin-like growth factor-1, or the neurotrophins (NGF and BDNF). Reduction in cellular Cholesterol also results in increased basal autophagy and impairment of induction of autophagy by glucose deprivation[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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In Vivo |
Cholesterol can be used in animal modeling to construct a rat hyperlipidemia model. The metabolic half-life of Cholesterol varies with the type of lipoprotein it binds and the tissue in which it is located, ranging from hours to years. Cholesterol can be used for hyperlipidemia, atherosclerosis and other related modeling purposes[4].The metabolic half-life of Cholesterol varies with the type of lipoprotein it binds to and the different tissues it is located in, ranging from a few hours to several years. Cholesterol can be used for modeling purposes related to hyperlipidemia and atherosclerosis[4]. 1. Induction of Hyperlipidemia[5][6] Background
Hyperlipidemia is a group of disorders characterized by elevated concentrations of circulating lipids, including cholesterol, cholesterol esters, phospholipids and triglycerides. If the intake of cholesterol is too much, and exceeds the body's metabolic capacity, it may lead to increased plasma cholesterol levels, causing hyperlipidemia.
Specific Mmodeling Methods
Rats: Wistar • male • 18-week-old (period: 8 weeks) Administration: 2% cholesterol; diet • 8 weeks
Note
(1) Rats were raised in a room with a room temperature of 22±2°C and a 12-h light-dark cycle.
(2) Serum cholesterol and triglyceride levels in Wistar rats are moderately increased by a high-cholesterol diet and do not develop substantial atherosclerosis. Wistar rats can be used in models of hyperlipidemia to study the direct effects of hyperlipidemia on the myocardium independent of atherosclerosis. Modeling Record
Molecular changes: Significant increase in total cholesterol levels in blood samples (about 20%) 2. Induction of atherosclerosis[7][8] Background
High levels of cholesterol in the blood, especially low-density lipoprotein cholesterol (LDL-C), can accumulate plaque on the walls of blood vessels, a process known as atherosclerosis. Over time, these plaques can block blood flow and cause serious health problems such as myocardial ischemia or myocardial infarction.
Specific Mmodeling Methods
Rabbits: Oryctolagus cuniculus • male • 4–6-month-old (period: 16 weeks) Administration: 0.3% cholesterol and 3% soybean oil; diet • 16 weeks
Note
(1) The cholesterol-fed rabbit is a widely used model for experimental atherosclerosis research as cholesterol only cause atherosclerotic changes in the rabbit arterial intima, which was very similar to human atherosclerosis. (2) As the absorption of dietary cholesterol requires fat, you must add oil into the diet. Otherwise, rabbits will use their internal fat, which makes them lean or sick. In addition, using soybean oil, which consists of unsaturated fatty acids, can prevent the levels of plasma cholesterol from becoming too high. Other vegetable oils, such as peanut oil or corn oil, can be used because they are all unsaturated fatty acids. Animal fat (saturated fatty acids) like tallow and lard is not recommended. (3) 0.3–0.5% cholesterol diet is recommended for most experiments. Rabbits cannot tolerate a 1–2% cholesterol diet for a month as they develop severe liver dysfunction. (4) Adult rabbits at 4 months or older can consume approximately ~150 g a day. You can either feed ab libitum or restricted (100–150 g/day/adult rabbit). (5) Plasma lipids should be measured weekly, especially for the first 4 weeks, because you need to determine whether plasma levels of cholesterol are elevated in each animal. Non-responder rabbits can be excluded from the experiments if their plasma cholesterol levels do not increase after cholesterol diet feeding. (6) Plasma lipoproteins can be measured at 8 and 16 weeks when the plasma levels of cholesterol are stable. (7) The age of rabbits should be considered because young rabbits are more susceptible to aortic atherosclerosis than old rabbits even though they have similar plasma cholesterol levels. 4–6-month-old rabbits are usually used for cholesterol feeding experiments. (8) Male and female rabbits are different in terms of response to a cholesterol diet and atherosclerosis. In our experience, female rabbits develop higher hypercholesterolemia and greater aortic lesions than their counterpart male rabbits. In general, male rabbits are recommended for experiments because estrogen may influence the results. Modeling Record
Histological changes: atherosclerosis lesions can be seen on HE stained aortic arch and thoracic aorta segments Correlated Product(s): Soybean oil (HY-108750) MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Clinical Trial |
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Molecular Weight |
386.65 |
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Formula |
C27H46O |
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CAS No. |
57-88-5 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3(18)[C@]2(18)CC[C@@]4(C)[C@@]3(18)CC[C@]4(18)[C@@H](CCCC(C)C)C |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
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Solvent & Solubility |
In Vitro:
Ethanol : 20 mg/mL (51.73 mM; Need ultrasonic) DMSO : < 1 mg/mL (insoluble or slightly soluble) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
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Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
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Purity & Documentation |
Purity: 99.94% |
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References |
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Complete Stock Solution Preparation Table
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Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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Ethanol | 1 mM | 2.5863 mL | 12.9316 mL | 25.8632 mL | 64.6580 mL |
5 mM | 0.5173 mL | 2.5863 mL | 5.1726 mL | 12.9316 mL | |
10 mM | 0.2586 mL | 1.2932 mL | 2.5863 mL | 6.4658 mL | |
15 mM | 0.1724 mL | 0.8621 mL | 1.7242 mL | 4.3105 mL | |
20 mM | 0.1293 mL | 0.6466 mL | 1.2932 mL | 3.2329 mL | |
25 mM | 0.1035 mL | 0.5173 mL | 1.0345 mL | 2.5863 mL | |
30 mM | 0.0862 mL | 0.4311 mL | 0.8621 mL | 2.1553 mL | |
40 mM | 0.0647 mL | 0.3233 mL | 0.6466 mL | 1.6164 mL | |
50 mM | 0.0517 mL | 0.2586 mL | 0.5173 mL | 1.2932 mL |
Cholesterol Related Classifications
- Metabolic Disease Cancer
- Cancer Immunotherapy Cancer Metabolism and Metastasis
- Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease Anti-infection
- Liposome Estrogen Receptor/ERR Endogenous Metabolite Bacterial