CF®594 Goat Anti-Human IgG (H&L), Fc Gamma Specific, highly cross adsorbed
Cat# 20446-50uL
Size : 50uL
Brand : Biotium
Goat Anti-Human IgG (H+L), Fc Gamma Specific, Highly Cross-Adsorbed
Highly cross-adsorbed goat anti-human IgG (H+L), Fc gamma specific secondary antibody labeled with our superior CF® dyes.
Technology
CF® Dyes
Considerations for Immunofluorescence Staining
Secondary Antibody Product Listings
Tech Tips
Protocol: Immunofluorescence Staining of Cells for Microscopy
Protocol: Fluorescent Western Blotting
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host
Supporting Documents
Product Description
This is a highly cross-adsorbed goat anti-human IgG (H L), Fc gamma specific secondary antibody labeled with our superior CF® dyes. The antibody reacts specifically with the human IgG heavy chains and not with light chains or F(ab’)2 fragment of human immunoglobulins. To minimize cross-reactivity, the antibodies are cross-adsorbed against bovine, horse, mouse and rabbit serum IgG prior to conjugation.
- Highly cross-adsorbed for specific staining with minimal background
- Available in 5 bright and photostable CF® dyes
- Suitable for western, immunofluorescence, and immunohistology
| Conjugation | Ex/Em | Size | Catalog No. | Dye Features |
|---|---|---|---|---|
| CF®488A | 490/515 nm | 50 uL (100 ug) | 20444-50uL | CF®488A Features |
| 0.5 mL (1 mg) | 20444-500uL | |||
| 1 mg (lyophilized) | 20444-1mg | |||
| CF®543 | 541/560 nm | 50 uL (100 ug) | 20445-50uL | CF®543 Features |
| 0.5 mL (1 mg) | 20445-500uL | |||
| 1 mg (lyophilized) | 20445-1mg | |||
| CF®594 | 593/614 nm | 50 uL (100 ug) | 20446-50uL | CF®594 Features |
| 0.5 mL (1 mg) | 20446-500uL | |||
| 1 mg (lyophilized) | 20446-1mg | |||
| CF®640R | 642/662 nm | 50 uL (100 ug) | 20447-50uL | CF®640R Features |
| 0.5 mL (1 mg) | 20447-500uL | |||
| 1 mg (lyophilized) | 20447-1mg | |||
| CF®647 | 650/665 nm | 50 uL (100 ug) | 20448-50uL | CF®647 Features |
| 0.5 mL (1 mg) | 20448-500uL | |||
| 1 mg (lyophilized) | 20448-1mg |
View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.
CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.
Storage and Handling
Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.
Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:
CF® Dye and biotin conjugates: add 0.5 mL dH2O
HRP or DNP conjugates: add 1 mL dH2O
Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.
Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.
Note: Storage of the antibody for more than a day at final working dilution is not recommended.
CF is a registered trademark of Biotium, Inc.
References
Download a list of CF® dye references.