Reagents and instruments for immunology, cell biology and molecular biology.

CD90 (Thy1.1) (APC)

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Cat# C2441-04A-APC-100ul

Size : 100ul

Brand : US Biological

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C2441-04A-APC CD90 (Thy1.1) (APC)

Clone Type

Polyclonal

Host

mouse

Source

rat

Isotype

IgG1

Grade

Affinity Purified

Applications

FC IF IHC IP

Crossreactivity

Gp Mo Rb Rt

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

The Thy-1 antigen is found on a variety of cell types including thymocytes, neuronal cells, T-Lymphocytes (mouse), stem cells and immature B cells (rat) and connective tissue.||Applications:|Suitable for use in Flow Cytometry, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested. ||Recommended Dilutions: |Immunohistochemistry: Frozen sections|Optimal dilution determined by the researcher. ||Hybridoma: |NS1 myeloma cells with spleen cells from Balb/c mice. ||Recommended Negative Control: |I1904-78Y: IgG1, Murine Negative Control (For rat tissue)||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: 6A341 (OX-7)|Host: mouse|Source: rat|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Rat Thy1 antigen. Swiss/UniProt Accession: P01830|Specificity: Recognizes rat CD90 also known as Thy1.1. Thy1.1 is a monomorphic determinant in rat but polymorphic in mice. Reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. Species Crossreactivity: mouse, guinea pig and rabbit||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Rat Thy1 antigen. Swiss/UniProt Accession: P01830

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

Purity

Purified by Protein G affinity chromatography from tissue culture supernatant.

Specificity

Recognizes rat CD90 also known as Thy1.1. Thy1.1 is a monomorphic determinant in rat but polymorphic in mice. Reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. Species Crossreactivity: mouse, guinea pig and rabbit

References

1. Mason, D.W. et al. (1980) Biochem.J. 187: 1-20. 2. Campbell, D.G. et al. (1981) Biochem. J. 195: 15-30. 3. Bukovský, A. et al. (1983) Immunology. 48:587-595. 4. Lee, W. et al. (1998) Circ. Res. 82: 845-851. 5. Jeng, C .J. et al. (1998) J. Cell. Biol. 140(3): 685-98. 6. Banerjee, S.A. et al. (1997) J. Neurosci. 17: 2756-2765.7. Kawachi, H. et al. (1992) Clin. Exp. Immunol. 88: 399-404.8. Tamura, M. et al. (1996) Biochem Biophys Res Comm.9. Stevenson, K.S. et al. (2009) Stem Cells Dev. 18:1389-98. 10. Biermann, J. et al. (2011) Mol Vis. 17: 395-403. 11. Keller, R.K. et al. (2004) J Lipid Res. 45: 347-5512. Ohashi, N. et al. (2010) Hypertens Res. 33:1174-81.