- More Files
Western Blot (Cell lysate)
Western blot analysis of BIRC5 in HeLa whole cell lysate (30 ug) using BIRC5 polyclonal antibody (Cat # PAB12470) at 1 ug/mL (1 minute exposure).
- Specifications
Product Description
Rabbit polyclonal antibody raised against full length recombinant BIRC5.
Immunogen
Recombinant protein corresponding to full length human BIRC5.
Host
Rabbit
Reactivity
Cat, Dog, Human, Mouse, Rat
Specificity
This antibody is specific to survivin.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ChIP (1:50-1:500)
Immunocytochemistry (1:50-1:500)
Immunofluorescence (1:50-1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:500)
Immunoprecipitation (1:1000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot (Cell lysate)
Western blot analysis of BIRC5 in HeLa whole cell lysate (30 ug) using BIRC5 polyclonal antibody (Cat # PAB12470) at 1 ug/mL (1 minute exposure).Immunofluorescence
- Gene Info — BIRC5
Entrez GeneID
332Protein Accession#
O15392Gene Name
BIRC5
Gene Alias
API4, EPR-1
Gene Description
baculoviral IAP repeat-containing 5
Omim ID
603352Gene Ontology
HyperlinkGene Summary
This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq
Other Designations
apoptosis inhibitor 4|baculoviral IAP repeat-containing protein 5|survivin variant 3 alpha
- Interactomes
- Pathways
- Diseases
- Publication Reference
- Mitotic deregulation by survivin in ErbB2-overexpressing breast cancer cells contributes to Taxol resistance.
Lu J, Tan M, Huang WC, Li P, Guo H, Tseng LM, Su XH, Yang WT, Treekitkarnmongkol W, Andreeff M, Symmans F, Yu D.
Clinical Cancer Research 2009 Feb; 15(4):1326.
- Impaired insulin-mediated vasorelaxation in diabetic Goto-Kakizaki rats is caused by impaired Akt phosphorylation.
Lee JH, Palaia T, Ragolia L.
American Journal of Physiology. Cell Physiology 2009 Feb; 296(2):C327.
- Molecularly targeted radiosensitization of human prostate cancer by modulating inhibitor of apoptosis.
Dai Y, Liu M, Tang W, DeSano J, Burstein E, Davis M, Pienta K, Lawrence T, Xu L.
Clinical Cancer Research 2008 Dec; 14(23):7701.
Application：WB-Ce, Human, DU-145, PC-3, PrEC, WI-38 cells.
- Cyclic nucleotide phosphodiesterase profiling reveals increased expression of phosphodiesterase 7B in chronic lymphocytic leukemia.
Zhang L, Murray F, Zahno A, Kanter JR, Chou D, Suda R, Fenlon M, Rassenti L, Cottam H, Kipps TJ, Insel PA.
PNAS 2008 Dec; 105(49):19532.
Application：WB-Ce, Human, CLL cell from patients with chronic lymphocytic leukemia (CLL).
- BKM1740, an acyl-tyrosine bisphosphonate amide derivative, inhibits the bone metastatic growth of human prostate cancer cells by inducing apoptosis.
Seo SI, Gera L, Zhau HE, Qian WP, Iqbal S, Johnson NA, Zhang S, Zayzafoon M, Stewart J, Wang R, Chung LW, Wu D.
Clinical Cancer Research 2008 Oct; 14(19):6198.
Application：IHC-P, WB, Human, Human prostate cancer, Human prostate tumor xenografts in mouse bone.
- Mitotic deregulation by survivin in ErbB2-overexpressing breast cancer cells contributes to Taxol resistance.