Bevacizumab (Avastin) Pharmacokinetic ELISA
Cat# EL-1611-021
Size : 1X96wells
Brand : AffinityImmuno
The Bevacizumab ELISA kit is designed to measure free Bevacizumab with high specificity and sensitivity, through use of a pair of antibodies.
INTRODUCTION
The Bevacizumab ELISA kit is designed to measure free Bevacizumab with high specificity and sensitivity. The assay design uses a pair of antibodies allowing detection of whole Bevacizumab molecules in biological matrices. Bevacizumab (Trade name Avastin®) is a recombinant humanized monoclonal antibody that blocks angiogenesis by inhibiting vascular endothelial growth factor A(VEGF-A). VEGF-A is a chemical signal that stimulates angiogenesis in a variety of diseases, especially in cancer. Bevacizumab was the first clinically available angiogenesis inhibitor in the United States.
PRINCIPLE OF THE ASSAY
This assay employs the sandwich enzyme immunoassay technique. Anti-Bevacizumab is coated onto a 96 well microplate. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Bevacizumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color devel opment is proportional to the amount of Bevaciuzumab present in test samples.
-
Catalog Number:
EL-1611-021 -
Application(s)
measure free Bevacizumab -
Target Protein
avastin -
Alternative Name of Target
bevacizumab -
Size
96 wells -
Sensitivity
detection limit 1.5 ng/mL -
Detection Method
Peroxidase / OD450 -
Precision
Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%. -
Detection Limit
1.5ng/ml -
Storage Conditions
Stable at -20°C for 1 year -
Safety Warnings and Precautions
Research Use Only. Follow instructions. -
Each Kit Includes
Coated microtiter plate, 96 wells Calibrator diluent. - 1.8ml Calibrator (250 ug/mL) - 12ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3
Product Specifications
-
Catalog Number:
EL-1611-021 -
Application(s)
measure free Bevacizumab -
Target Protein
avastin -
Alternative Name of Target
bevacizumab -
Size
96 wells -
Sensitivity
detection limit 1.5 ng/mL -
Detection Method
Peroxidase / OD450 -
Precision
Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%. -
Detection Limit
1.5ng/ml -
Storage Conditions
Stable at -20°C for 1 year -
Safety Warnings and Precautions
Research Use Only. Follow instructions. -
Each Kit Includes
Coated microtiter plate, 96 wells Calibrator diluent. - 1.8ml Calibrator (250 ug/mL) - 12ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3