Reagents and instruments for immunology, cell biology and molecular biology.

Anti-RPLP1 antibody (1-114)

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Cat# NB-22-23197-200

Size : 200uL(2x100uL)

Brand : Neo Biotech

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Immunofluorescence analysis of MCF7 cells using RPLP1 Rabbit polyclonal antibody . Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of various lysates using RPLP1 Rabbit polyclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

General Info

Host:	Rabbit
Applications:	WB/IF
Reactivity:	Human/Mouse/Rat/Zebrafish
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-RPLP1 (1-114) is suitable for use in Western Blot and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IF/ICC 1:50-1:100
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	RPLP1
Gene ID:	6176
Uniprot ID:	RLA1_HUMAN
Immunogen Region:	1-114
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human RPLP1 (NP_000994.1).
Immunogen Sequence:	MASVSELACIYSALILHDDE VTVTEDKINALIKAAGVNVE PFWPGLFAKALANVNIGSLI CNVGAGGPAPAAGAAPAGGP APSTAAAPAEEKKVEAKKEE SEESDDDMGFGLFD

Description

Post Translational Modifications	Ubiquitinated at Lys-92 and Lys-93 by RNF14 and RNF25 in response to ribosome collisions (ribosome stalling).
Function	Plays an important role in the elongation step of protein synthesis.
Protein Name	Large Ribosomal Subunit Protein P1 60s Acidic Ribosomal Protein P1
Database Links	Reactome: R-HSA-156827 Reactome: R-HSA-156902 Reactome: R-HSA-1799339 Reactome: R-HSA-192823 Reactome: R-HSA-2408557 Reactome: R-HSA-6791226 Reactome: R-HSA-72689 Reactome: R-HSA-72706 Reactome: R-HSA-72764 Reactome: R-HSA-9010553 Reactome: R-HSA-9633012 Reactome: R-HSA-975956 Reactome: R-HSA-975957
Alternative Antibody Names	Anti-Large Ribosomal Subunit Protein P1 antibody Anti-60s Acidic Ribosomal Protein P1 antibody Anti-RPLP1 antibody Anti-RRP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance