Reagents and instruments for immunology, cell biology and molecular biology.

Anti-BCL2 antibody (1-100 aa) (Clone: 6B5)

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Cat# NB-22-6941-50

Size : 50ug

Brand : Neo Biotech

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Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Western blot analysis of lysates from 1) Hela, 2) MCF-7 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.
Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Western blot detection of Bcl-2 in human breast cancer cell line MCF-7 (A), MDA-MB-231 (B) and Cal51 (C) using Bcl-2 mouse mAb ( .Predicted band size: 26kDa.Observed band size:26kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital
Immunohistochemical analysis of paraffin-embedded Human breast cancer. 1, Using Bcl-2 Mouse mAb ( diluted at 1:200 (4°C overnight). 2, High-pressure and temperature Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 50min). Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital
Western blot analysis of chicken cell lysis using Antibody diluted at 1:1000
IHC staining of Human tonsil tissue paraffin-embedded, diluted at 1:200.
Western blot analysis of Hela, diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunofluorescence analysis of Hela cell. 1, Dnmt3b Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Bcl-2 monoclonal antibody (6B5) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

General Info

Host:	Mouse
Applications:	IF/WB/IHC
Reactivity:	Human/Mouse/Rat/Chicken
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Mouse monoclonal antibody anti-Apoptosis regulator Bcl-2 (1-100 aa) is suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry research applications.

Clonality:	Monoclonal
Clone ID:	6B5
Conjugation:	Unconjugated
Isotype:	IgG1
Formulation:	Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration:	0.73 mg/mL
Dilution Range:	IF 1:50-200 WB 1:1000~2000 IHC 1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	BCL2
Gene ID:	596
Uniprot ID:	BCL2_HUMAN
Immunogen Region:	1-100 aa
Specificity:	The antibody detects endogenous Bcl-2 proteins.
Immunogen:	Synthetic Peptide of human Bcl-2 at the amino acid range 1-100

Description

Post Translational Modifications	Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophag. Dephosphorylated by protein phosphatase 2A (PP2A). Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. Monoubiquitinated by PRKN, leading to an increase in its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome. Ubiquitinated by XIAP, leading to its degradation by the proteasome.
Function	Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function. May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release.
Protein Name	Apoptosis Regulator Bcl-2
Database Links	Reactome: R-HSA-111447 Reactome: R-HSA-111453 Reactome: R-HSA-6785807 Reactome: R-HSA-844455 Reactome: R-HSA-9018519 Reactome: R-HSA-9634638
Cellular Localisation	Mitochondrion Outer Membrane Single-Pass Membrane Protein Nucleus Membrane Endoplasmic Reticulum Membrane Cytoplasm
Alternative Antibody Names	Anti-Apoptosis Regulator Bcl-2 antibody Anti-BCL2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance