AM4-64
Cat# 70025
Size : 1MG
Brand : Biotium
AM4-64
AM4-64 is a fixable, activity-dependent fluorescent nerve terminal probe. AM4-64 functions similarly similarly as SynaptoRed™ C2 (#70021) (equivalent to FM®4-64), but with the additional property of being fixable.
Technology
Membrane Potential & Nerve Terminal Dyes
Neuronal Probes and Tools
Protocols
SDS
Supporting Documents
Product Description
AM4-64 is a fixable, activity-dependent fluorescent nerve terminal probe. AM4-64 functions similarly as SynaptoRed™ C2 (70021) (equivalent to FM®4-64), but with the additional property of being fixable. Thus, AM4-64 is a useful tool for synapse studies where subsequent fluorescent immunochemistry is desired. AM4-64 and FM®4-64 have identical excitation and emission wavelengths.
- λEx/λEm (membranes) = ~510/750 nm*
- Dark purple solid soluble in water
- MW: 555.5
* Excitation/emission settings of 515/640 nm have been used for detection of yeast vacuole staining with SynaptoRed™ C2 (FM®4-64), and should be applicable to AM4-64.
Nerve Terminal Dyes and Kits
| Product | Catalog No. | Features |
|---|---|---|
| SynaptoGreen™ Dyes (Ex/Em ~480/660 nm in membranes) | 70020... 70053 | View full list of SynaptoGreen™ Dyes |
| SynaptoRed Dyes™ (Ex/Em ~510/750 nm in membranes) | 70021... 70050 | View full list of SynaptoRed™ Dyes |
| Background Reducers | ||
| ADVASEP-7 | 70029 | Sulfonated cyclodextrin that aids in removal of free dye during washes |
| SCAS | 70037 | Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7 |
| Sulforhodamine 101 | 80101 | Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes |
| Nerve Terminal Staining Kits | ||
| Nerve Terminal Staining Kit I | 70030 | Includes SynaptoGreen™ C4 and ADVASEP-7 |
| Nerve Terminal Staining Kit II (A) | 70031 | Includes AM1-43 and ADVASEP-7 |
| Nerve Terminal Staining Kit II (B) | 70031-1 | Includes AM1-43 and SCAS |
| Nerve Terminal Staining Kit III | 70032 | Includes SynaptoGreen™ C4 and Sulforhodamine 101 |
| Nerve Terminal Staining Kit V | 70034 | Includes SynaptoRed™ C2 and ADVASEP-7 |
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
FM is a registered trademark of Thermo Fisher Scientific.