Adipogenesis Fluorometric Assay Kit
Cat# E-BC-F065-96T
Size : 96T
Brand : Elabscience
| Detection Principle | Triglycerides were converted by enzyme to produce hydrolytic products, which were catalyzed by the enzyme to produce fluorescent substances. The triglyceride accumulation can be calculated by meas-uring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm. |
| Sample Type | Tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Energy Metabolism |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 1.4 μmol/L |
| Detection Range | 1.4-32 μmol/L |
| Precision | inter-assay CV: 8.4% | intra-assay CV: 0.04 |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Porcine heart tissue homogenate | 3-6 |
| 10% Porcine liver tissue homogenate | 8-10 |
| CHO cell (10^6) | 1 |
| 293T cell (10^6) | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
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Other Formats
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor®405
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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