(R)-Sulforaphane [142825-10-3]
Cat# HY-13755A-10mg
Size : 10mg
Brand : MedChemExpress
(R)-Sulforaphane (L-Sulforaphane) is a orally active, potent inducer of the Keap1/Nrf2/ARE pathway, exhibiting antioxidant and anticancer activities. (R)-Sulforaphane primarily functions by upregulating phase II detoxifying enzymes in cells, aiding in the removal of carcinogens and combating oxidative stress. (R)-Sulforaphane is capable of modulating gene expression, influencing various signaling pathways, including Nrf2, NF-κB, and AP-1. (R)-Sulforaphane can be used in studies of tumor biology, antioxidant defense mechanisms, as well as inflammation and immune responses.
For research use only. We do not sell to patients.
(R)-Sulforaphane Chemical Structure
CAS No. : 142825-10-3
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Other Forms of (R)-Sulforaphane:
- Sulforaphane In-stock
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Description |
(R)-Sulforaphane (L-Sulforaphane) is a orally active, potent inducer of the Keap1/Nrf2/ARE pathway, exhibiting antioxidant and anticancer activities. (R)-Sulforaphane primarily functions by upregulating phase II detoxifying enzymes in cells, aiding in the removal of carcinogens and combating oxidative stress. (R)-Sulforaphane is capable of modulating gene expression, influencing various signaling pathways, including Nrf2, NF-κB, and AP-1. (R)-Sulforaphane can be used in studies of tumor biology, antioxidant defense mechanisms, as well as inflammation and immune responses[1][2][3][4][5][6][7]. |
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IC50 & Target |
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In Vitro |
(R)-Sulforaphane (10 μM, 24 hours) pre-treatment of H9c2 cells reduces the number of apoptotic cells, decreases the expression of pro-apoptotic proteins (Bax, caspase-3, cytochrome c), and counteracts the increase in mitochondrial membrane potential induced by Doxorubicin (HY-15142A) (1 μM, 2 hours)[4]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot Analysis[5]
Apoptosis Analysis[4]
RT-PCR[4]
Western Blot Analysis[4]
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Molecular Weight |
177.29 |
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Formula |
C6H11NOS2 |
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CAS No. |
142825-10-3 |
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Appearance |
Liquid |
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Color |
Colorless to light yellow |
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SMILES |
O=[S@@](CCCCN=C=S)C |
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Structure Classification |
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Initial Source |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
-20°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
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Solvent & Solubility |
In Vitro:
DMSO : 100 mg/mL (564.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 99.37% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 5.6405 mL | 28.2024 mL | 56.4048 mL | 141.0119 mL |
5 mM | 1.1281 mL | 5.6405 mL | 11.2810 mL | 28.2024 mL | |
10 mM | 0.5640 mL | 2.8202 mL | 5.6405 mL | 14.1012 mL | |
15 mM | 0.3760 mL | 1.8802 mL | 3.7603 mL | 9.4008 mL | |
20 mM | 0.2820 mL | 1.4101 mL | 2.8202 mL | 7.0506 mL | |
25 mM | 0.2256 mL | 1.1281 mL | 2.2562 mL | 5.6405 mL | |
30 mM | 0.1880 mL | 0.9401 mL | 1.8802 mL | 4.7004 mL | |
40 mM | 0.1410 mL | 0.7051 mL | 1.4101 mL | 3.5253 mL | |
50 mM | 0.1128 mL | 0.5640 mL | 1.1281 mL | 2.8202 mL | |
60 mM | 0.0940 mL | 0.4700 mL | 0.9401 mL | 2.3502 mL | |
80 mM | 0.0705 mL | 0.3525 mL | 0.7051 mL | 1.7626 mL | |
100 mM | 0.0564 mL | 0.2820 mL | 0.5640 mL | 1.4101 mL |
(R)-Sulforaphane Related Classifications
- NF-κB Apoptosis Metabolic Enzyme/Protease Immunology/Inflammation
- Keap1-Nrf2 Bcl-2 Family Caspase Reactive Oxygen Species NF-κB