(AP) Broad Spectrum (FastRed) kit, 1 Kit (8 ml/80 tests)
Cat# orb1087641
Size : 1unit
Brand : Biorbyt
(AP) Broad Spectrum (FastRed) kit, 1 Kit (8 ml/80 tests)
Catalog Number: orb1087641
| Catalog Number | orb1087641 |
|---|---|
| Category | Assays and Kits |
| Description | (AP) Broad Spectrum (FastRed) kit, 1 Kit (8 ml/80 tests) |
| Tested applications | IF, IHC-Fr, IHC-P |
| Immunogen | Please contact us for more information. |
| Storage | The solution should be stored at 2-8°C without further dilution. Please store the reagent in a dark place and do not freeze it. Under these conditions the solution is stable up to the expiry date. It should not be used after the expiry date. A positive and a negative control have to be carried out in parallel to the test material. If you observe unusual staining or other deviations from the expected results which could possibly be caused by the kit reagents, please contact our technical support . |
| Note | For research use only |
| Application notes | Paraffin-embedded tissue sections are first deparaffinised and rehydrated. Background staining caused by unspecific binding of the primary or secondary antibody is minimized via incubation with a protein blocking solution (“Blocking Solution” provided with the kit). This step can be omitted if the primary antibodies are diluted in an appropriate buffer. The next step is incubation with the specific primary antibody. After washing, the biotinylated secondary antibody is applied and incubated. This secondary antibody functions as a link between primary antibody and streptavidin-alkaline phosphatase-conjugate (“Streptavidin-AP-Conjugate“). A second washing is followed by the application of this conjugate. It binds to biotin at the secondary antibody. Any excess of unbound streptavidin-AP-conjugate is thoroughly washed away after incubation. The addition of the chromogenic substrate starts the enzymatic reaction of the alkaline phosphatase which leads to colour precipitation where the primary antibody is bound. The colour can be observed with a light microscope. The chromogen used determines the colour. The chromogen Permanent Red leads to the formation of a magenta-red product of reaction at the place of the target antigen. Other suitable chromogens are Permanent AP Red (magenta-red) or NBT (blue-black) with its substrate BCIP. |
| Expiration Date | Please enquire. |